Review of the article „Trace Extraction of Metoprolol from Plasma, Urine and EBC samples Using Modified Magnetic Nanoparticles Followed by Spectrofluorimetric Determination for Therapeutic Drug Monitoring Purposes“, verified by Publons, Web of Science
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Abstract: Background: Metoprolol is a selective β1-adrenergic receptor
antagonists (β -blockers). It is widely used for the treatment of hypertension
and other related diseases. Metoprolol can be used as doping agent in sport,
thus has been added to the list of forbidden drugs. In Iran, therapeutic drug
monitoring (TDM) of beta-blockers is an applied procedure in some cases.
Therapeutic regimen could be easily managed by determination of drug
levels in biological fluids which is relatively costly process and requires high
skilled technical staff. Using a simple and low-cost analytical procedure may
help to use TDM in routine clinical practice.
Method: A real biological sample was prepared and adjusted to pH 3-4, then
metoprolol was quickly extracted using iron oxide magnetic nanoparticles
(MNPs) modified by the surfactant sodium dodecyl sulfate (SDS) and
determined applying spectrofluorimetry at 340 ± 3 nm after excitation at 283
± 3 nm.
Results: The extraction and determ...ination conditions including, the amount
of NPs and SDS, pH of solution, standing time and desorption solvent type
and volume were investigated and adjusted and optimized. Calibration
curves were linear over the concentration range 6–100 ng/ml for plasma and
5–100 ng/ml for water, urine and exhaled breath condensate (EBC) samples,
respectively. Intra and inter-day precision values for metoprolol in different
samples were less than 5.6 and 6%, respectively, and accuracy (as relativeerror) was better than 5%. Standard addition recovery tests were carried out,
and the analytical recoveries ranged from 86% to 113%. The limits of
detection (LOD) and quantification (LOQ) of metoprolol were found to be in
the range of 2.1-3.4 and 6.3-10.2 ng/ml, respectively.
Conclusion: The developed method was successfully applied to one
volunteer with hypertension who had been given an oral tablet of 50 mg
metoprolol.
Source:
Current Pharmaceutical Analysis, 2019, 1/BMS-CPA-2018-254-48Collections
Institution/Community
Mašinski fakultetTY - JOUR AU - Jovanović, Tamara PY - 2019 UR - https://machinery.mas.bg.ac.rs/handle/123456789/5959 AB - Abstract: Background: Metoprolol is a selective β1-adrenergic receptor antagonists (β -blockers). It is widely used for the treatment of hypertension and other related diseases. Metoprolol can be used as doping agent in sport, thus has been added to the list of forbidden drugs. In Iran, therapeutic drug monitoring (TDM) of beta-blockers is an applied procedure in some cases. Therapeutic regimen could be easily managed by determination of drug levels in biological fluids which is relatively costly process and requires high skilled technical staff. Using a simple and low-cost analytical procedure may help to use TDM in routine clinical practice. Method: A real biological sample was prepared and adjusted to pH 3-4, then metoprolol was quickly extracted using iron oxide magnetic nanoparticles (MNPs) modified by the surfactant sodium dodecyl sulfate (SDS) and determined applying spectrofluorimetry at 340 ± 3 nm after excitation at 283 ± 3 nm. Results: The extraction and determination conditions including, the amount of NPs and SDS, pH of solution, standing time and desorption solvent type and volume were investigated and adjusted and optimized. Calibration curves were linear over the concentration range 6–100 ng/ml for plasma and 5–100 ng/ml for water, urine and exhaled breath condensate (EBC) samples, respectively. Intra and inter-day precision values for metoprolol in different samples were less than 5.6 and 6%, respectively, and accuracy (as relativeerror) was better than 5%. Standard addition recovery tests were carried out, and the analytical recoveries ranged from 86% to 113%. The limits of detection (LOD) and quantification (LOQ) of metoprolol were found to be in the range of 2.1-3.4 and 6.3-10.2 ng/ml, respectively. Conclusion: The developed method was successfully applied to one volunteer with hypertension who had been given an oral tablet of 50 mg metoprolol. T2 - Current Pharmaceutical Analysis T1 - Review of the article „Trace Extraction of Metoprolol from Plasma, Urine and EBC samples Using Modified Magnetic Nanoparticles Followed by Spectrofluorimetric Determination for Therapeutic Drug Monitoring Purposes“, verified by Publons, Web of Science EP - 48 SP - 1/BMS-CPA-2018-254 UR - https://hdl.handle.net/21.15107/rcub_machinery_5959 ER -
@article{ author = "Jovanović, Tamara", year = "2019", abstract = "Abstract: Background: Metoprolol is a selective β1-adrenergic receptor antagonists (β -blockers). It is widely used for the treatment of hypertension and other related diseases. Metoprolol can be used as doping agent in sport, thus has been added to the list of forbidden drugs. In Iran, therapeutic drug monitoring (TDM) of beta-blockers is an applied procedure in some cases. Therapeutic regimen could be easily managed by determination of drug levels in biological fluids which is relatively costly process and requires high skilled technical staff. Using a simple and low-cost analytical procedure may help to use TDM in routine clinical practice. Method: A real biological sample was prepared and adjusted to pH 3-4, then metoprolol was quickly extracted using iron oxide magnetic nanoparticles (MNPs) modified by the surfactant sodium dodecyl sulfate (SDS) and determined applying spectrofluorimetry at 340 ± 3 nm after excitation at 283 ± 3 nm. Results: The extraction and determination conditions including, the amount of NPs and SDS, pH of solution, standing time and desorption solvent type and volume were investigated and adjusted and optimized. Calibration curves were linear over the concentration range 6–100 ng/ml for plasma and 5–100 ng/ml for water, urine and exhaled breath condensate (EBC) samples, respectively. Intra and inter-day precision values for metoprolol in different samples were less than 5.6 and 6%, respectively, and accuracy (as relativeerror) was better than 5%. Standard addition recovery tests were carried out, and the analytical recoveries ranged from 86% to 113%. The limits of detection (LOD) and quantification (LOQ) of metoprolol were found to be in the range of 2.1-3.4 and 6.3-10.2 ng/ml, respectively. Conclusion: The developed method was successfully applied to one volunteer with hypertension who had been given an oral tablet of 50 mg metoprolol.", journal = "Current Pharmaceutical Analysis", title = "Review of the article „Trace Extraction of Metoprolol from Plasma, Urine and EBC samples Using Modified Magnetic Nanoparticles Followed by Spectrofluorimetric Determination for Therapeutic Drug Monitoring Purposes“, verified by Publons, Web of Science", pages = "48-1/BMS-CPA-2018-254", url = "https://hdl.handle.net/21.15107/rcub_machinery_5959" }
Jovanović, T.. (2019). Review of the article „Trace Extraction of Metoprolol from Plasma, Urine and EBC samples Using Modified Magnetic Nanoparticles Followed by Spectrofluorimetric Determination for Therapeutic Drug Monitoring Purposes“, verified by Publons, Web of Science. in Current Pharmaceutical Analysis, 1/BMS-CPA-2018-254-48. https://hdl.handle.net/21.15107/rcub_machinery_5959
Jovanović T. Review of the article „Trace Extraction of Metoprolol from Plasma, Urine and EBC samples Using Modified Magnetic Nanoparticles Followed by Spectrofluorimetric Determination for Therapeutic Drug Monitoring Purposes“, verified by Publons, Web of Science. in Current Pharmaceutical Analysis. 2019;:1/BMS-CPA-2018-254-48. https://hdl.handle.net/21.15107/rcub_machinery_5959 .
Jovanović, Tamara, "Review of the article „Trace Extraction of Metoprolol from Plasma, Urine and EBC samples Using Modified Magnetic Nanoparticles Followed by Spectrofluorimetric Determination for Therapeutic Drug Monitoring Purposes“, verified by Publons, Web of Science" in Current Pharmaceutical Analysis (2019):1/BMS-CPA-2018-254-48, https://hdl.handle.net/21.15107/rcub_machinery_5959 .