Review of the article „Simultaneous Determination of Eight Potential Q-Markers in Zishen Tongguan Capsules Based on UHPLC-MS/MS“, verified by Publons, Web of Science
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Abstract
Background: Zishen Tongguan (ZSTG) Capsule was a hospital preparation of Affiliated Hospital of Nanjing University of TCM ,and has been clinically proven to be effective for treating pyelonephritis and benign prostatic hyperplasia. However, its quality standards are not perfect; a comprehensive study of the “quality marker” (Q-marker) of this medicine has not been carried out.
Experimental Methods: In this paper, a sensitive and specific ultra-high performance liquid chromatographic-tandem mass spectrometric (UHPLC–MS/MS) method was developed for the simultaneous determination of eight potential Q-Markers of ZSTG, including Timosaponin A3, Berberine hydrochloride, Jatrorrhizine, Phellodendrine, Palmatine hydrochloride, Mangiferin, Neomangiferin, and Timosaponin BII. Kromasil 100-3.5 C18 column, mobile phase is a mixture of 0.2% formic acid with acetonitrile by gradient elution at a flow rate of 0.2 mL/min achieved in 13 minutes was used for separation. Detection was performe...d on a positive/negative mode with multiple-reaction-monitoring (MRM).
Results: The analytical condition was validated in terms of the linearity, accuracy and precision, repeatability, and stability. The method established in this assay was successfully applied to the study of the potential Q-Markers in 8 batches of commercial samples, which supported the potential of this method for improving the quality control of ZSTG.
Conclusion: It is the first time to report the rapid and simultaneous analysis of eight Q-Markers in ZSTG by UHPLC-MS/MS and apply to determine 8 batches of ZSTG 3 This method could be considered as good quality criteria to control the quality of ZSTG.
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Current Pharmaceutical Analysis, 2019, 1/BMS-CPA-2019-3-29Колекције
Институција/група
Mašinski fakultetTY - JOUR AU - Jovanović, Tamara PY - 2019 UR - https://machinery.mas.bg.ac.rs/handle/123456789/5947 AB - Abstract Background: Zishen Tongguan (ZSTG) Capsule was a hospital preparation of Affiliated Hospital of Nanjing University of TCM ,and has been clinically proven to be effective for treating pyelonephritis and benign prostatic hyperplasia. However, its quality standards are not perfect; a comprehensive study of the “quality marker” (Q-marker) of this medicine has not been carried out. Experimental Methods: In this paper, a sensitive and specific ultra-high performance liquid chromatographic-tandem mass spectrometric (UHPLC–MS/MS) method was developed for the simultaneous determination of eight potential Q-Markers of ZSTG, including Timosaponin A3, Berberine hydrochloride, Jatrorrhizine, Phellodendrine, Palmatine hydrochloride, Mangiferin, Neomangiferin, and Timosaponin BII. Kromasil 100-3.5 C18 column, mobile phase is a mixture of 0.2% formic acid with acetonitrile by gradient elution at a flow rate of 0.2 mL/min achieved in 13 minutes was used for separation. Detection was performed on a positive/negative mode with multiple-reaction-monitoring (MRM). Results: The analytical condition was validated in terms of the linearity, accuracy and precision, repeatability, and stability. The method established in this assay was successfully applied to the study of the potential Q-Markers in 8 batches of commercial samples, which supported the potential of this method for improving the quality control of ZSTG. Conclusion: It is the first time to report the rapid and simultaneous analysis of eight Q-Markers in ZSTG by UHPLC-MS/MS and apply to determine 8 batches of ZSTG 3 This method could be considered as good quality criteria to control the quality of ZSTG. T2 - Current Pharmaceutical Analysis T1 - Review of the article „Simultaneous Determination of Eight Potential Q-Markers in Zishen Tongguan Capsules Based on UHPLC-MS/MS“, verified by Publons, Web of Science EP - 29 SP - 1/BMS-CPA-2019-3 UR - https://hdl.handle.net/21.15107/rcub_machinery_5947 ER -
@article{ author = "Jovanović, Tamara", year = "2019", abstract = "Abstract Background: Zishen Tongguan (ZSTG) Capsule was a hospital preparation of Affiliated Hospital of Nanjing University of TCM ,and has been clinically proven to be effective for treating pyelonephritis and benign prostatic hyperplasia. However, its quality standards are not perfect; a comprehensive study of the “quality marker” (Q-marker) of this medicine has not been carried out. Experimental Methods: In this paper, a sensitive and specific ultra-high performance liquid chromatographic-tandem mass spectrometric (UHPLC–MS/MS) method was developed for the simultaneous determination of eight potential Q-Markers of ZSTG, including Timosaponin A3, Berberine hydrochloride, Jatrorrhizine, Phellodendrine, Palmatine hydrochloride, Mangiferin, Neomangiferin, and Timosaponin BII. Kromasil 100-3.5 C18 column, mobile phase is a mixture of 0.2% formic acid with acetonitrile by gradient elution at a flow rate of 0.2 mL/min achieved in 13 minutes was used for separation. Detection was performed on a positive/negative mode with multiple-reaction-monitoring (MRM). Results: The analytical condition was validated in terms of the linearity, accuracy and precision, repeatability, and stability. The method established in this assay was successfully applied to the study of the potential Q-Markers in 8 batches of commercial samples, which supported the potential of this method for improving the quality control of ZSTG. Conclusion: It is the first time to report the rapid and simultaneous analysis of eight Q-Markers in ZSTG by UHPLC-MS/MS and apply to determine 8 batches of ZSTG 3 This method could be considered as good quality criteria to control the quality of ZSTG.", journal = "Current Pharmaceutical Analysis", title = "Review of the article „Simultaneous Determination of Eight Potential Q-Markers in Zishen Tongguan Capsules Based on UHPLC-MS/MS“, verified by Publons, Web of Science", pages = "29-1/BMS-CPA-2019-3", url = "https://hdl.handle.net/21.15107/rcub_machinery_5947" }
Jovanović, T.. (2019). Review of the article „Simultaneous Determination of Eight Potential Q-Markers in Zishen Tongguan Capsules Based on UHPLC-MS/MS“, verified by Publons, Web of Science. in Current Pharmaceutical Analysis, 1/BMS-CPA-2019-3-29. https://hdl.handle.net/21.15107/rcub_machinery_5947
Jovanović T. Review of the article „Simultaneous Determination of Eight Potential Q-Markers in Zishen Tongguan Capsules Based on UHPLC-MS/MS“, verified by Publons, Web of Science. in Current Pharmaceutical Analysis. 2019;:1/BMS-CPA-2019-3-29. https://hdl.handle.net/21.15107/rcub_machinery_5947 .
Jovanović, Tamara, "Review of the article „Simultaneous Determination of Eight Potential Q-Markers in Zishen Tongguan Capsules Based on UHPLC-MS/MS“, verified by Publons, Web of Science" in Current Pharmaceutical Analysis (2019):1/BMS-CPA-2019-3-29, https://hdl.handle.net/21.15107/rcub_machinery_5947 .