Review of the article „Development an Efficient and Sensitive Magnetic Dispersive Solid Phase Microextraction Technique for Preconcentration of Amphetamine and Methamphetamine Determined by High-Performance Liquid Chromatography and Liquid Chromatography-Tandem Mass Spectrometry in Dietary Supplements“, verified by Publons, Web of Science

Abstract

Abstract Background: The sport and weigh management supplements are prime target for adulteration. Amphetamines are widely known to cause appetite suppression and encourage weight loss. Amphetamines also abused by athletes to improve their performance. Objective: A rapid and simple magnetic dispersive solid phase extraction method was proposed in order to isolate amphetamine (AM) and methamphetamine (MET) from complex dietary supplements. Method: A high-performance liquid chromatography-ultraviolet (HPLC-UV) method with precision and accuracy better than 10% was proposed to detect AM and MET in the range of 300-1500 ng/ml and 500-2000 ng/ml, respectively. Results: Confirmation study of AM and MET was accomplished by ion-spray LC-MS/MS using multiple reaction monitoring (MRM) mode. The LC-MS/MS method was validated in the range of 5-100 ng/ml and 1-200 ng/ml for AM and MET, respectively. Conclusion: Sixteen dietary supplements comprising both weight management and sport supplements were tested with the developed methods. AM and MET were detected in four samples.