Review of the article „The Metabolisms of Tanshinone IIA Protocatechuic aldehyde, Danshensu, Salvianolic acid B and Hydroxysafflor yellow A in Zebrafish“, verified by Publons, Web of Science

Abstract

Mean centering, double divisor, ratio spectra-zero crossing, and successive derivative were applied for estimation of metformin, empagliflozin, and glimepiride in the concentration range of 1.0-10, 2.5-30, and 1.0-10 µgmL-1, respectively in their prepared laboratory mixtures and in pharmaceutical tablets, without prior chemical separation. In some cases, lifestyle changes aren’t enough to keep type 2 diabetes under control, there are several medications that may help. Metformin, can lower your blood sugar levels. Glimepiride, make more insulin. Empagliflozin, prevent the kidneys from reabsorbing sugar into the blood and sending it out in urine. The absorption spectra of these drugs were recorded in the range of 200-400nm, mean centering for metformin were measured at 232 and 244 nm, empagliflozin and glimepiride had amplitude values at 262 and 278nm, respectively. The derivative of double divisor was measured at 234, 278, and 288nm for metformin, empagliflozine and glimepiride, respectively. The ratio spectra-zero crossing were quantifying at the amplitude values of analytical signal at 234 and 274nm for metformin and empagliflozine, respectively, whereas glimepiride was determined at 242 and 286nm. The successive ratio of metformin, empagliflozin, and glimepiride were determined at 284, 242, and 266nm, respectively. The methods were studies and optimized, upon the validation linearity, precision, accuracy LOD, LOQ and selectivity were proved to be effective for analysis of the mentioned drugs in pharmaceutical dosage form. Statistical comparison done between the proposed methods with the reported methods with respect to accuracy and precision no significant difference was found by student’s t-test, F-test and one-way ANOVA.