Review of the article „Pharmacokinetic differences of grape seed procyanidins by gavage administration between Alzheimerˈs Disease and normal in rats“, verified by Publons, Web of Science
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ABSTRACT: Background: Grape Seed Procyanidins(GSP) is a kind of natural polyphenols with strong antioxidant capacity. Studies have shown that GSP have a
significant effect in the alleviation of Alzheimer's Disease (AD).
Objective: In order to investigate whether there is a pharmacokinetics difference
in GSP between normal and AD rats, a rapid UPLC-MS/MS method for detecting the
content of it in plasma samples was established, and we analyzed the plasma
concentrations of procyanidin B2, procyanidin B3, catechin and epicatechin in normal
and AD rats over time to determine the plasma concentration of GSP.
Methods: We used 400μL of methanol for the protein precipitation solvent in the
plasma treatment. The chromatographic separation was performed on a C18 column at
20 . The mobile phase was a gradient of acid water ℃ and methanol containing 0.1%
(v/v) formic acid, time was 15min.
Results: From the analysis of the experimental results, it can be seen that the plasma
concentrati...ons of procyanidin B2, procyanidin B3, catechin and epicatechin in AD rats
were significantly higher than those in normal rats (P < 0.05). And after comparison,
it also can be seen that the content of epicatechin was the highest in rats given
procyanidin, followed by catechin, procyanidin B2, procyanidin B3.
Conclusion: We found that the absorptions of these analytes in AD group were more
than in normal group, provides an analytical basis for the treatment of AD with
procyanidins.
Key words: Grape seed procyanidins, UPLC-MS/MS, pharmacokinetics, Alzheimer's
disease, plasma concentration, gavage administration
Извор:
Current Pharmaceutical Analysis, 2019, 1/BMS-CPA-2019-84-19Колекције
Институција/група
Mašinski fakultetTY - JOUR AU - Jovanović, Tamara PY - 2019 UR - https://machinery.mas.bg.ac.rs/handle/123456789/5891 AB - ABSTRACT: Background: Grape Seed Procyanidins(GSP) is a kind of natural polyphenols with strong antioxidant capacity. Studies have shown that GSP have a significant effect in the alleviation of Alzheimer's Disease (AD). Objective: In order to investigate whether there is a pharmacokinetics difference in GSP between normal and AD rats, a rapid UPLC-MS/MS method for detecting the content of it in plasma samples was established, and we analyzed the plasma concentrations of procyanidin B2, procyanidin B3, catechin and epicatechin in normal and AD rats over time to determine the plasma concentration of GSP. Methods: We used 400μL of methanol for the protein precipitation solvent in the plasma treatment. The chromatographic separation was performed on a C18 column at 20 . The mobile phase was a gradient of acid water ℃ and methanol containing 0.1% (v/v) formic acid, time was 15min. Results: From the analysis of the experimental results, it can be seen that the plasma concentrations of procyanidin B2, procyanidin B3, catechin and epicatechin in AD rats were significantly higher than those in normal rats (P < 0.05). And after comparison, it also can be seen that the content of epicatechin was the highest in rats given procyanidin, followed by catechin, procyanidin B2, procyanidin B3. Conclusion: We found that the absorptions of these analytes in AD group were more than in normal group, provides an analytical basis for the treatment of AD with procyanidins. Key words: Grape seed procyanidins, UPLC-MS/MS, pharmacokinetics, Alzheimer's disease, plasma concentration, gavage administration T2 - Current Pharmaceutical Analysis T1 - Review of the article „Pharmacokinetic differences of grape seed procyanidins by gavage administration between Alzheimerˈs Disease and normal in rats“, verified by Publons, Web of Science EP - 19 SP - 1/BMS-CPA-2019-84 UR - https://hdl.handle.net/21.15107/rcub_machinery_5891 ER -
@article{ author = "Jovanović, Tamara", year = "2019", abstract = "ABSTRACT: Background: Grape Seed Procyanidins(GSP) is a kind of natural polyphenols with strong antioxidant capacity. Studies have shown that GSP have a significant effect in the alleviation of Alzheimer's Disease (AD). Objective: In order to investigate whether there is a pharmacokinetics difference in GSP between normal and AD rats, a rapid UPLC-MS/MS method for detecting the content of it in plasma samples was established, and we analyzed the plasma concentrations of procyanidin B2, procyanidin B3, catechin and epicatechin in normal and AD rats over time to determine the plasma concentration of GSP. Methods: We used 400μL of methanol for the protein precipitation solvent in the plasma treatment. The chromatographic separation was performed on a C18 column at 20 . The mobile phase was a gradient of acid water ℃ and methanol containing 0.1% (v/v) formic acid, time was 15min. Results: From the analysis of the experimental results, it can be seen that the plasma concentrations of procyanidin B2, procyanidin B3, catechin and epicatechin in AD rats were significantly higher than those in normal rats (P < 0.05). And after comparison, it also can be seen that the content of epicatechin was the highest in rats given procyanidin, followed by catechin, procyanidin B2, procyanidin B3. Conclusion: We found that the absorptions of these analytes in AD group were more than in normal group, provides an analytical basis for the treatment of AD with procyanidins. Key words: Grape seed procyanidins, UPLC-MS/MS, pharmacokinetics, Alzheimer's disease, plasma concentration, gavage administration", journal = "Current Pharmaceutical Analysis", title = "Review of the article „Pharmacokinetic differences of grape seed procyanidins by gavage administration between Alzheimerˈs Disease and normal in rats“, verified by Publons, Web of Science", pages = "19-1/BMS-CPA-2019-84", url = "https://hdl.handle.net/21.15107/rcub_machinery_5891" }
Jovanović, T.. (2019). Review of the article „Pharmacokinetic differences of grape seed procyanidins by gavage administration between Alzheimerˈs Disease and normal in rats“, verified by Publons, Web of Science. in Current Pharmaceutical Analysis, 1/BMS-CPA-2019-84-19. https://hdl.handle.net/21.15107/rcub_machinery_5891
Jovanović T. Review of the article „Pharmacokinetic differences of grape seed procyanidins by gavage administration between Alzheimerˈs Disease and normal in rats“, verified by Publons, Web of Science. in Current Pharmaceutical Analysis. 2019;:1/BMS-CPA-2019-84-19. https://hdl.handle.net/21.15107/rcub_machinery_5891 .
Jovanović, Tamara, "Review of the article „Pharmacokinetic differences of grape seed procyanidins by gavage administration between Alzheimerˈs Disease and normal in rats“, verified by Publons, Web of Science" in Current Pharmaceutical Analysis (2019):1/BMS-CPA-2019-84-19, https://hdl.handle.net/21.15107/rcub_machinery_5891 .