Review of the article “Development of Two Innovative 96-Microwell-Based Spectrophotometric Assays with High Throughput for Determination of Fluoroquinolone Antibiotics in their Pharmaceutical Formulations“, verified by Publons, Web of Science
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Abstract: Background: Fluoroquinolone antibiotics (FQAs) have broad-spectrum anti-bacterial activity, high potency, variable indications, and excellent pharmacokinetic profiles. Many spectrophotometric assays have been described for determination of FQAs in their pharmaceutical formulations; however, most of these assays have limited throughputs to be applied in quality control laboratories.
Objectives: This study was devoted to the development and validation of two innovative 96-micorwell-based spectrophotometric assays with high throughput for pharmaceuticals quality control of seven fluoroquinolone antibiotics (FQAs).
Methods: These FQAs were levofloxacin (LEV), norfloxacin (NOR), ciprofloxacin (CIP), gemifloxacin (GEM), danofloxacin (DAN), enrofloxacin (ENR) and marbofloxacin (MAR). The first assay (assay I) was developed for LEV, NOR, CIP, and GEM via formation of red-colored metal complexes (MC) with FeCl3. The second assay (assay II) was developed for LEV, DAN, ENR, and MAR v...ia formation of red-colored charge transfer complexes (CTC) with 2,3-dich1oro-5,6-dicyano-1,4-benzoquinone (DDQ). The reactions of FQAs with both FeCl3 and DDQ reagents were carried out in transparent 96-microwell plates and the absorbances of the colored-complexes were measured by absorbance microwell plate reader at 460 nm.
Results: The optimum reaction conditions were established for both assays; under which, Beer’s law correlating the absorbances with the concentrations of FQAs were obeyed in the range of 10 – 100 µg/well with good correlation coefficients (0.9943 – 0.9982). The limits of detection were in the range of 4.5 – 7.5 µg/well, and the limits of quantification were in the range of 14.9 – 25.0 µg/well. Both assays showed high precisions as the values of the relative standard deviations (RSD) did not exceed 3.4%. The accuracy of both assays was proved by recovery studies as the recovery values were in the range of 98.1 – 102.6% (± 0.9 – 2.7%). The proposed assays were applied successfully for the determination of all FQAs in their tablets with good accuracy and precisions.
Conclusion: The proposed assays are simple, economic, and more importantly have high throughput; therefore, they are convenient and valuable for routine use for in pharmaceutical quality control laboratories for determination of FQAs in pharmaceutical formulations. An additional advantage of the proposed assays is that all the FQAs could be determined on a single system without modifications in the detection wavelength.
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Current Pharmaceutical Analysis, 2021, 1/BMS-CPA-2020-310-27Collections
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Mašinski fakultetTY - JOUR AU - Jovanović, Tamara PY - 2021 UR - https://machinery.mas.bg.ac.rs/handle/123456789/5855 AB - Abstract: Background: Fluoroquinolone antibiotics (FQAs) have broad-spectrum anti-bacterial activity, high potency, variable indications, and excellent pharmacokinetic profiles. Many spectrophotometric assays have been described for determination of FQAs in their pharmaceutical formulations; however, most of these assays have limited throughputs to be applied in quality control laboratories. Objectives: This study was devoted to the development and validation of two innovative 96-micorwell-based spectrophotometric assays with high throughput for pharmaceuticals quality control of seven fluoroquinolone antibiotics (FQAs). Methods: These FQAs were levofloxacin (LEV), norfloxacin (NOR), ciprofloxacin (CIP), gemifloxacin (GEM), danofloxacin (DAN), enrofloxacin (ENR) and marbofloxacin (MAR). The first assay (assay I) was developed for LEV, NOR, CIP, and GEM via formation of red-colored metal complexes (MC) with FeCl3. The second assay (assay II) was developed for LEV, DAN, ENR, and MAR via formation of red-colored charge transfer complexes (CTC) with 2,3-dich1oro-5,6-dicyano-1,4-benzoquinone (DDQ). The reactions of FQAs with both FeCl3 and DDQ reagents were carried out in transparent 96-microwell plates and the absorbances of the colored-complexes were measured by absorbance microwell plate reader at 460 nm. Results: The optimum reaction conditions were established for both assays; under which, Beer’s law correlating the absorbances with the concentrations of FQAs were obeyed in the range of 10 – 100 µg/well with good correlation coefficients (0.9943 – 0.9982). The limits of detection were in the range of 4.5 – 7.5 µg/well, and the limits of quantification were in the range of 14.9 – 25.0 µg/well. Both assays showed high precisions as the values of the relative standard deviations (RSD) did not exceed 3.4%. The accuracy of both assays was proved by recovery studies as the recovery values were in the range of 98.1 – 102.6% (± 0.9 – 2.7%). The proposed assays were applied successfully for the determination of all FQAs in their tablets with good accuracy and precisions. Conclusion: The proposed assays are simple, economic, and more importantly have high throughput; therefore, they are convenient and valuable for routine use for in pharmaceutical quality control laboratories for determination of FQAs in pharmaceutical formulations. An additional advantage of the proposed assays is that all the FQAs could be determined on a single system without modifications in the detection wavelength. T2 - Current Pharmaceutical Analysis T1 - Review of the article “Development of Two Innovative 96-Microwell-Based Spectrophotometric Assays with High Throughput for Determination of Fluoroquinolone Antibiotics in their Pharmaceutical Formulations“, verified by Publons, Web of Science EP - 27 SP - 1/BMS-CPA-2020-310 UR - https://hdl.handle.net/21.15107/rcub_machinery_5855 ER -
@article{ author = "Jovanović, Tamara", year = "2021", abstract = "Abstract: Background: Fluoroquinolone antibiotics (FQAs) have broad-spectrum anti-bacterial activity, high potency, variable indications, and excellent pharmacokinetic profiles. Many spectrophotometric assays have been described for determination of FQAs in their pharmaceutical formulations; however, most of these assays have limited throughputs to be applied in quality control laboratories. Objectives: This study was devoted to the development and validation of two innovative 96-micorwell-based spectrophotometric assays with high throughput for pharmaceuticals quality control of seven fluoroquinolone antibiotics (FQAs). Methods: These FQAs were levofloxacin (LEV), norfloxacin (NOR), ciprofloxacin (CIP), gemifloxacin (GEM), danofloxacin (DAN), enrofloxacin (ENR) and marbofloxacin (MAR). The first assay (assay I) was developed for LEV, NOR, CIP, and GEM via formation of red-colored metal complexes (MC) with FeCl3. The second assay (assay II) was developed for LEV, DAN, ENR, and MAR via formation of red-colored charge transfer complexes (CTC) with 2,3-dich1oro-5,6-dicyano-1,4-benzoquinone (DDQ). The reactions of FQAs with both FeCl3 and DDQ reagents were carried out in transparent 96-microwell plates and the absorbances of the colored-complexes were measured by absorbance microwell plate reader at 460 nm. Results: The optimum reaction conditions were established for both assays; under which, Beer’s law correlating the absorbances with the concentrations of FQAs were obeyed in the range of 10 – 100 µg/well with good correlation coefficients (0.9943 – 0.9982). The limits of detection were in the range of 4.5 – 7.5 µg/well, and the limits of quantification were in the range of 14.9 – 25.0 µg/well. Both assays showed high precisions as the values of the relative standard deviations (RSD) did not exceed 3.4%. The accuracy of both assays was proved by recovery studies as the recovery values were in the range of 98.1 – 102.6% (± 0.9 – 2.7%). The proposed assays were applied successfully for the determination of all FQAs in their tablets with good accuracy and precisions. Conclusion: The proposed assays are simple, economic, and more importantly have high throughput; therefore, they are convenient and valuable for routine use for in pharmaceutical quality control laboratories for determination of FQAs in pharmaceutical formulations. An additional advantage of the proposed assays is that all the FQAs could be determined on a single system without modifications in the detection wavelength.", journal = "Current Pharmaceutical Analysis", title = "Review of the article “Development of Two Innovative 96-Microwell-Based Spectrophotometric Assays with High Throughput for Determination of Fluoroquinolone Antibiotics in their Pharmaceutical Formulations“, verified by Publons, Web of Science", pages = "27-1/BMS-CPA-2020-310", url = "https://hdl.handle.net/21.15107/rcub_machinery_5855" }
Jovanović, T.. (2021). Review of the article “Development of Two Innovative 96-Microwell-Based Spectrophotometric Assays with High Throughput for Determination of Fluoroquinolone Antibiotics in their Pharmaceutical Formulations“, verified by Publons, Web of Science. in Current Pharmaceutical Analysis, 1/BMS-CPA-2020-310-27. https://hdl.handle.net/21.15107/rcub_machinery_5855
Jovanović T. Review of the article “Development of Two Innovative 96-Microwell-Based Spectrophotometric Assays with High Throughput for Determination of Fluoroquinolone Antibiotics in their Pharmaceutical Formulations“, verified by Publons, Web of Science. in Current Pharmaceutical Analysis. 2021;:1/BMS-CPA-2020-310-27. https://hdl.handle.net/21.15107/rcub_machinery_5855 .
Jovanović, Tamara, "Review of the article “Development of Two Innovative 96-Microwell-Based Spectrophotometric Assays with High Throughput for Determination of Fluoroquinolone Antibiotics in their Pharmaceutical Formulations“, verified by Publons, Web of Science" in Current Pharmaceutical Analysis (2021):1/BMS-CPA-2020-310-27, https://hdl.handle.net/21.15107/rcub_machinery_5855 .