Review of the article “Permeation of Hydroxypropyl-Beta-Cyclodextrin and Its Inclusion Complex through Mouse Small Intestine, Determined by a Spectrophotometry“, verified by Publons, Web of Science

Abstract

Abstract Background: Cyclodextrins (CDs) are commonly used host molecules of inclusion complex. However, due to the lack of sensitive method to determine CDs, the absorption process of CDs remains unclear. Objective: In this study, oleuropein (OL) inclusion complex employing hydroxylpropyl-beta-cyclodextrin (HP-beta-CD) as host molecules was prepared and the formation of inclusion complex was ascertained by FT-IR and DSC. A spectrophotometry was established for the determination of HP-beta-CD, based on the fact that the absorbance of phenolphthalein (PP) decreased in the presence of HP-beta-CD Methods: The assay conditions were optimized to augment the method sensitivity. Molecular docking was employed to verify the strong interaction between PP and HP-beta-CD. The permeation process of free HP-beta-CD, HP-beta-CD of OL inclusion complex, free OL, and OL in the inclusion complex, was examined, respectively, using an in vitro mouse small intestine model. Results: Though HP-beta-CD possessed hydrophilic outside shell, it could permeate through small intestine quickly with cumulative permeating amount over 90% in 2 h. Free HP-beta-CD, the host molecule HP-beta-CD, and guest molecule OL of the inclusion complex exhibited the consistent permeating profiles across mouse small intestine. Conclusion: The approach for the determination of HP-beta-CD was accurate and precise (%RSD=2.98). During the transportation process, HP-beta-CD may hold OL in the cavity and deliver it across small intestine.