Review of the article Preparation of Molecularly Imprinted Polymer for Selective Extraction of Spiked Cloprostenol from Milk Samples

Abstract

Aims: This study was conducted to develop a particular sorbent for the selective extraction of the luteolytic drug cloprostenol from milk samples. Background: Cloprostenol's luteolytic activity has also been proved in animal, whereas the production of prostaglandins is discussed extensively in the literature, while extraction of cloprostenol is rarely discussed. A careful study of the literature reveals that there is no previously reported molecularly imprinted polymer or method of extraction for cloprostenol from milk samples. Objective: The major objective was to get a sufficient amount of molecularly imprinted polymers (MIPs) for selective extraction and detection of cloprostenol. After a series of experiment, MIPs and their NIPs, which are analogues of MIPs, were synthesized. Method: In these experiments, radical polymerization with non-covalent interactions has been used to prepare MIPs and their analogues. Different parameters to determine the optimization conditions such as solvent volume, cross-linker and ratio of template were studied in various sets to get better possible characteristics of MIPs. Result: The HPLC has been used to evaluate the retention capacity which ranges from 68.5% to 94.1%. The mechanism of adsorption was studied by the isothermal assay and kinetic studies. The kinetic studies exhibited the high retention capacity within 20 minutes of MIPs contact. The percentage of polymer yield ranged from 53.5 % to 92.3 %. The High-performance liquid chromatography coupled with UV detector showed the limit of detection and limit of quantification 30.5 ng/mL and 86.7ng/mL, respectively, for determination of analytes from milk samples. The recovery of cloprostenol for all spiked samples of milk was higher than 88%. Conclusion: Furthermore, the studies showed that MIPs are specific in the adsorption of cloprostenol compared to its other structural analogues. The outcomes also showed the significance of cross-linker concentration and amount of solvent during the process of polymerization. These parameters greatly affect the preorganization of complementary functional groups, meanwhile it creates specific cavities for the targeted drugs. Others: The studies showed that MIPs are specific in adsorption to cloprostenol compared to its other structural analogues.