Review of the article Mature Wistar Rats' Spleen and Liver Histomorphological Changes Related to the Duration of Atrazine Exposure, verified by Publons, Web of Science
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Abstract
Background Spleen and liver is secondary lymphoid organ that is highly sensitive to different chemicals. Widespread use of pesticides in agriculture has always been a matter of concern. And surprisingly, atrazine distinguishes out for being used more frequently among numerous harmful pesticides. As a result, longterm exposure to atrazine and other pesticides is thought to produce metabolic abnormalities; however, little is known about how atrazine affects the spleen and liver and how this relates to its histoachitectural structure.
Aim: The histopathology of the spleen and liver from rats exposed to atrazine was the subject of our investigation.
Materials and Methods: Twenty (20) male wistar rats ranging from150-200g were acclimated to laboratory conditions for 14days, following which they were randomly assigned into 4 groups 1, 2, 3 and 4 of 5 animals each based on average body weight. Groups (2-4) were administered atrazine via oral route corresponding to 1237 mg/kg (20/...5 LD50), 618 mg/kg (10/5 LD50) and 309 mg/Kg/body weight (1/10 LD50) for 7, 14 and 30days, while group I (control) received distilled water orally using orogastric canula for 30days . The liver and spleen from each group of rats were havested, weighed, and fixed in 10% buffered formal saline fixative before being taken for histological examination 24 hours following the experimental periods of oral administration of the extract.
Results: At the end of the experiment, the histological findings showed increased and numerious area of the white pulp of spleen from rats exposed to atrazine as compared to that from the control. The relative area of germinal centre in the structure of the splenic lymph follicles of rats exposed to atrazine also revealed increased. Also, Histopathologically, the liver showed necrotic hepatic cells and congested central vein, with the highest atrazine concentration causing the most adverse effects.
Conclusion: Our data demonstrated that rats exposed to high-dose of atrazine led to hypertrophy of white pulp of the spleen and hepatic cell damage with liver. From this we concluded that both organ are highly sensitive to the debilitating effects of atrazine
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Asian Journal of Research in Medical and Pharmaceutical Sciences, 2022, 1/Ms_AJRIMPS_94460-10Колекције
Институција/група
Mašinski fakultetTY - JOUR AU - Jovanović, Tamara PY - 2022 UR - https://machinery.mas.bg.ac.rs/handle/123456789/5662 AB - Abstract Background Spleen and liver is secondary lymphoid organ that is highly sensitive to different chemicals. Widespread use of pesticides in agriculture has always been a matter of concern. And surprisingly, atrazine distinguishes out for being used more frequently among numerous harmful pesticides. As a result, longterm exposure to atrazine and other pesticides is thought to produce metabolic abnormalities; however, little is known about how atrazine affects the spleen and liver and how this relates to its histoachitectural structure. Aim: The histopathology of the spleen and liver from rats exposed to atrazine was the subject of our investigation. Materials and Methods: Twenty (20) male wistar rats ranging from150-200g were acclimated to laboratory conditions for 14days, following which they were randomly assigned into 4 groups 1, 2, 3 and 4 of 5 animals each based on average body weight. Groups (2-4) were administered atrazine via oral route corresponding to 1237 mg/kg (20/5 LD50), 618 mg/kg (10/5 LD50) and 309 mg/Kg/body weight (1/10 LD50) for 7, 14 and 30days, while group I (control) received distilled water orally using orogastric canula for 30days . The liver and spleen from each group of rats were havested, weighed, and fixed in 10% buffered formal saline fixative before being taken for histological examination 24 hours following the experimental periods of oral administration of the extract. Results: At the end of the experiment, the histological findings showed increased and numerious area of the white pulp of spleen from rats exposed to atrazine as compared to that from the control. The relative area of germinal centre in the structure of the splenic lymph follicles of rats exposed to atrazine also revealed increased. Also, Histopathologically, the liver showed necrotic hepatic cells and congested central vein, with the highest atrazine concentration causing the most adverse effects. Conclusion: Our data demonstrated that rats exposed to high-dose of atrazine led to hypertrophy of white pulp of the spleen and hepatic cell damage with liver. From this we concluded that both organ are highly sensitive to the debilitating effects of atrazine T2 - Asian Journal of Research in Medical and Pharmaceutical Sciences T1 - Review of the article Mature Wistar Rats' Spleen and Liver Histomorphological Changes Related to the Duration of Atrazine Exposure, verified by Publons, Web of Science EP - 10 SP - 1/Ms_AJRIMPS_94460 UR - https://hdl.handle.net/21.15107/rcub_machinery_5662 ER -
@article{ author = "Jovanović, Tamara", year = "2022", abstract = "Abstract Background Spleen and liver is secondary lymphoid organ that is highly sensitive to different chemicals. Widespread use of pesticides in agriculture has always been a matter of concern. And surprisingly, atrazine distinguishes out for being used more frequently among numerous harmful pesticides. As a result, longterm exposure to atrazine and other pesticides is thought to produce metabolic abnormalities; however, little is known about how atrazine affects the spleen and liver and how this relates to its histoachitectural structure. Aim: The histopathology of the spleen and liver from rats exposed to atrazine was the subject of our investigation. Materials and Methods: Twenty (20) male wistar rats ranging from150-200g were acclimated to laboratory conditions for 14days, following which they were randomly assigned into 4 groups 1, 2, 3 and 4 of 5 animals each based on average body weight. Groups (2-4) were administered atrazine via oral route corresponding to 1237 mg/kg (20/5 LD50), 618 mg/kg (10/5 LD50) and 309 mg/Kg/body weight (1/10 LD50) for 7, 14 and 30days, while group I (control) received distilled water orally using orogastric canula for 30days . The liver and spleen from each group of rats were havested, weighed, and fixed in 10% buffered formal saline fixative before being taken for histological examination 24 hours following the experimental periods of oral administration of the extract. Results: At the end of the experiment, the histological findings showed increased and numerious area of the white pulp of spleen from rats exposed to atrazine as compared to that from the control. The relative area of germinal centre in the structure of the splenic lymph follicles of rats exposed to atrazine also revealed increased. Also, Histopathologically, the liver showed necrotic hepatic cells and congested central vein, with the highest atrazine concentration causing the most adverse effects. Conclusion: Our data demonstrated that rats exposed to high-dose of atrazine led to hypertrophy of white pulp of the spleen and hepatic cell damage with liver. From this we concluded that both organ are highly sensitive to the debilitating effects of atrazine", journal = "Asian Journal of Research in Medical and Pharmaceutical Sciences", title = "Review of the article Mature Wistar Rats' Spleen and Liver Histomorphological Changes Related to the Duration of Atrazine Exposure, verified by Publons, Web of Science", pages = "10-1/Ms_AJRIMPS_94460", url = "https://hdl.handle.net/21.15107/rcub_machinery_5662" }
Jovanović, T.. (2022). Review of the article Mature Wistar Rats' Spleen and Liver Histomorphological Changes Related to the Duration of Atrazine Exposure, verified by Publons, Web of Science. in Asian Journal of Research in Medical and Pharmaceutical Sciences, 1/Ms_AJRIMPS_94460-10. https://hdl.handle.net/21.15107/rcub_machinery_5662
Jovanović T. Review of the article Mature Wistar Rats' Spleen and Liver Histomorphological Changes Related to the Duration of Atrazine Exposure, verified by Publons, Web of Science. in Asian Journal of Research in Medical and Pharmaceutical Sciences. 2022;:1/Ms_AJRIMPS_94460-10. https://hdl.handle.net/21.15107/rcub_machinery_5662 .
Jovanović, Tamara, "Review of the article Mature Wistar Rats' Spleen and Liver Histomorphological Changes Related to the Duration of Atrazine Exposure, verified by Publons, Web of Science" in Asian Journal of Research in Medical and Pharmaceutical Sciences (2022):1/Ms_AJRIMPS_94460-10, https://hdl.handle.net/21.15107/rcub_machinery_5662 .