Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism
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2017
Authors
Radić, DankaPavlović, Vera P.
Lazović, Milana M.
Jovičić-Petrović, Jelena P.
Karličić, Vera
Lalević, Blažo
Raičević, Vera
Article (Published version)
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Show full item recordAbstract
Modern, efficient and cost-effective approach to remediation of heavy metal-contaminated soil is based on the application of microorganisms. In this paper, four isolates from agricultural and urban contaminated soil showed abundant growth in the presence of copper(II) sulfate pentahydrate (CuSO4·5H2O) up to 2 mM. Selected yeasts were identified by molecular methods as Candida tropicalis (three isolates) and Schwanniomyces occidentalis (one isolate). C. tropicalis (4TD1101S) showed the highest percentage of bioaccumulation capabilities (94.37%), determined by the inductively coupled plasma optical emission spectrometry (ICP-OES). The Raman spectra of C. tropicalis (4TD1101S) analyzed in a medium with the addition of 2 mM CuSO4·5H2O showed certain increase in metallothionein production, which represents a specific response of the yeast species to the stress conditions. These results indicate that soil yeasts represent a potential for practical application in the bioremediation of contami...nated environments.
Keywords:
Yeasts / Raman spectra / Copper / Candida tropicalis / Bioremediation / BioaccumulationSource:
Environmental Science and Pollution Research, 2017, 24, 27, 21885-21893Publisher:
- Springer Heidelberg, Heidelberg
Funding / projects:
- Biodiversity as potential in ecoremediation technologies of degraded ecosystems (RS-MESTD-Technological Development (TD or TR)-31080)
- Directed synthesis, structure and properties of multifunctional materials (RS-MESTD-Basic Research (BR or ON)-172057)
DOI: 10.1007/s11356-017-9817-4
ISSN: 0944-1344
PubMed: 28779342
WoS: 000409962200034
Scopus: 2-s2.0-85026813698
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Mašinski fakultetTY - JOUR AU - Radić, Danka AU - Pavlović, Vera P. AU - Lazović, Milana M. AU - Jovičić-Petrović, Jelena P. AU - Karličić, Vera AU - Lalević, Blažo AU - Raičević, Vera PY - 2017 UR - https://machinery.mas.bg.ac.rs/handle/123456789/2570 AB - Modern, efficient and cost-effective approach to remediation of heavy metal-contaminated soil is based on the application of microorganisms. In this paper, four isolates from agricultural and urban contaminated soil showed abundant growth in the presence of copper(II) sulfate pentahydrate (CuSO4·5H2O) up to 2 mM. Selected yeasts were identified by molecular methods as Candida tropicalis (three isolates) and Schwanniomyces occidentalis (one isolate). C. tropicalis (4TD1101S) showed the highest percentage of bioaccumulation capabilities (94.37%), determined by the inductively coupled plasma optical emission spectrometry (ICP-OES). The Raman spectra of C. tropicalis (4TD1101S) analyzed in a medium with the addition of 2 mM CuSO4·5H2O showed certain increase in metallothionein production, which represents a specific response of the yeast species to the stress conditions. These results indicate that soil yeasts represent a potential for practical application in the bioremediation of contaminated environments. PB - Springer Heidelberg, Heidelberg T2 - Environmental Science and Pollution Research T1 - Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism EP - 21893 IS - 27 SP - 21885 VL - 24 DO - 10.1007/s11356-017-9817-4 ER -
@article{ author = "Radić, Danka and Pavlović, Vera P. and Lazović, Milana M. and Jovičić-Petrović, Jelena P. and Karličić, Vera and Lalević, Blažo and Raičević, Vera", year = "2017", abstract = "Modern, efficient and cost-effective approach to remediation of heavy metal-contaminated soil is based on the application of microorganisms. In this paper, four isolates from agricultural and urban contaminated soil showed abundant growth in the presence of copper(II) sulfate pentahydrate (CuSO4·5H2O) up to 2 mM. Selected yeasts were identified by molecular methods as Candida tropicalis (three isolates) and Schwanniomyces occidentalis (one isolate). C. tropicalis (4TD1101S) showed the highest percentage of bioaccumulation capabilities (94.37%), determined by the inductively coupled plasma optical emission spectrometry (ICP-OES). The Raman spectra of C. tropicalis (4TD1101S) analyzed in a medium with the addition of 2 mM CuSO4·5H2O showed certain increase in metallothionein production, which represents a specific response of the yeast species to the stress conditions. These results indicate that soil yeasts represent a potential for practical application in the bioremediation of contaminated environments.", publisher = "Springer Heidelberg, Heidelberg", journal = "Environmental Science and Pollution Research", title = "Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism", pages = "21893-21885", number = "27", volume = "24", doi = "10.1007/s11356-017-9817-4" }
Radić, D., Pavlović, V. P., Lazović, M. M., Jovičić-Petrović, J. P., Karličić, V., Lalević, B.,& Raičević, V.. (2017). Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism. in Environmental Science and Pollution Research Springer Heidelberg, Heidelberg., 24(27), 21885-21893. https://doi.org/10.1007/s11356-017-9817-4
Radić D, Pavlović VP, Lazović MM, Jovičić-Petrović JP, Karličić V, Lalević B, Raičević V. Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism. in Environmental Science and Pollution Research. 2017;24(27):21885-21893. doi:10.1007/s11356-017-9817-4 .
Radić, Danka, Pavlović, Vera P., Lazović, Milana M., Jovičić-Petrović, Jelena P., Karličić, Vera, Lalević, Blažo, Raičević, Vera, "Copper-tolerant yeasts: Raman spectroscopy in determination of bioaccumulation mechanism" in Environmental Science and Pollution Research, 24, no. 27 (2017):21885-21893, https://doi.org/10.1007/s11356-017-9817-4 . .