TY  - JOUR
AU  - Koruga, Djuro
AU  - Stanković, Ivana
AU  - Matija, Lidija
AU  - Khun, Dietmar
AU  - Christ, Bastien
AU  - Dembski, Sofia
AU  - Jevtic, Nenad
AU  - Janac, Jelena
AU  - Pavlovic, Vladimir
AU  - De Wever, Bart
PY  - 2024
UR  - https://machinery.mas.bg.ac.rs/handle/123456789/7763
AB  - In order to maximally reduce the toxicity of fullerenol (the first derivative of C60, FD-C60), and increase its biomedical efficiency, the second derivative SD-C60 (3HFWC, Hyper-Harmonized Hydroxylated Fullerene Water Complex) was created. Several different methods were applied in the comparative characterization of FD-C60 and SD-C60 with the same OH groups in their core. FD-C60 as an individual structure was about 1.3 nm in size, while SD-C60 as an individual structure was 10–30 nm in size. Based on ten physicochemical methods and techniques, FD-C60 and SD-C60 were found to be two different substances in terms of size, structure, and physicochemical properties; FD-C60, at 100 °C, had endothermic characteristics, while SD-C60, at 133 °C, had exothermic characteristics; FD-C60 did not have water layers, while SD-C60 had water layers; the zeta potential of FD-C60 was −25.85 mV, while it was −43.29 mV for SD-C60. SD-C60 is a promising substance for use in cosmetics and pharmaceuticals.
T2  - Nanomaterials
T1  - Comparative Studies of the Structural and Physicochemical Properties of the First Fullerene Derivative FD-C60 (Fullerenol) and Second Fullerene Derivate SD-C60 (3HFWC)
VL  - 5
DO  - 10.3390/nano14050480
ER  - 

@article{
author = "Koruga, Djuro and Stanković, Ivana and Matija, Lidija and Khun, Dietmar and Christ, Bastien and Dembski, Sofia and Jevtic, Nenad and Janac, Jelena and Pavlovic, Vladimir and De Wever, Bart",
year = "2024",
abstract = "In order to maximally reduce the toxicity of fullerenol (the first derivative of C60, FD-C60), and increase its biomedical efficiency, the second derivative SD-C60 (3HFWC, Hyper-Harmonized Hydroxylated Fullerene Water Complex) was created. Several different methods were applied in the comparative characterization of FD-C60 and SD-C60 with the same OH groups in their core. FD-C60 as an individual structure was about 1.3 nm in size, while SD-C60 as an individual structure was 10–30 nm in size. Based on ten physicochemical methods and techniques, FD-C60 and SD-C60 were found to be two different substances in terms of size, structure, and physicochemical properties; FD-C60, at 100 °C, had endothermic characteristics, while SD-C60, at 133 °C, had exothermic characteristics; FD-C60 did not have water layers, while SD-C60 had water layers; the zeta potential of FD-C60 was −25.85 mV, while it was −43.29 mV for SD-C60. SD-C60 is a promising substance for use in cosmetics and pharmaceuticals.",
journal = "Nanomaterials",
title = "Comparative Studies of the Structural and Physicochemical Properties of the First Fullerene Derivative FD-C60 (Fullerenol) and Second Fullerene Derivate SD-C60 (3HFWC)",
volume = "5",
doi = "10.3390/nano14050480"
}

Koruga, D., Stanković, I., Matija, L., Khun, D., Christ, B., Dembski, S., Jevtic, N., Janac, J., Pavlovic, V.,& De Wever, B.. (2024). Comparative Studies of the Structural and Physicochemical Properties of the First Fullerene Derivative FD-C60 (Fullerenol) and Second Fullerene Derivate SD-C60 (3HFWC). in Nanomaterials, 5.
https://doi.org/10.3390/nano14050480

Koruga D, Stanković I, Matija L, Khun D, Christ B, Dembski S, Jevtic N, Janac J, Pavlovic V, De Wever B. Comparative Studies of the Structural and Physicochemical Properties of the First Fullerene Derivative FD-C60 (Fullerenol) and Second Fullerene Derivate SD-C60 (3HFWC). in Nanomaterials. 2024;5.
doi:10.3390/nano14050480 .

Koruga, Djuro, Stanković, Ivana, Matija, Lidija, Khun, Dietmar, Christ, Bastien, Dembski, Sofia, Jevtic, Nenad, Janac, Jelena, Pavlovic, Vladimir, De Wever, Bart, "Comparative Studies of the Structural and Physicochemical Properties of the First Fullerene Derivative FD-C60 (Fullerenol) and Second Fullerene Derivate SD-C60 (3HFWC)" in Nanomaterials, 5 (2024),
https://doi.org/10.3390/nano14050480 . .

TY  - JOUR
AU  - Matija, Lidija
AU  - Stanković, Ivana
AU  - Purić, Milica
AU  - Miličić, Milica
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Krajnović, Tamara
AU  - Gordić, Vuk
AU  - Koruga, Đuro
PY  - 2023
UR  - https://machinery.mas.bg.ac.rs/handle/123456789/7203
AB  - The human body contains 60–70% water, depending on age. As a body fluid, it is not only a medium in which physical and chemical processes take place, but it is also one of the active mediators. Water is the richest substance with non-covalent hydrogen bonds. Water molecules, by themselves (in vacuum), are diamagnetic but when organized into clusters, they become diamagnetic or paramagnetic. Also, biomolecules (DNA, collagen, clathrin, and other proteins) have non-covalent hydrogen bonds in their structure. The interaction, as well as signal transmission, between water and biomolecules is achieved through the vibrations of covalent and non-covalent hydrogen bonds, which determine the state and dynamics of conformational changes in biomolecules. Disruptive conformational changes in biomolecules, cells, and tissues lead to their dysfunctionality, so they are a frequent cause of many disorders and diseases. For example, the rearrangement of hydrogen bonding due to mitochondrial disease mutation in cytochrome bc1 disturbs heme bH redox potential and spin state. In order to prevent and repair the dysfunctional conformational changes, a liquid substance was developed based on the second derivative of the C60 molecule (SD-C60), which has classical and quantum properties. The characterization of SD-C60 by UV-VIS-NIR, FTIR, TEM, and AFM/MFM was performed and it is shown that SD-C60 water layers generate vibrations with near-zero phase dispersion which are transmitted through Fibonacci’s water chains to biomolecules. In comparison with previously published SD-C60 derivate (3HFWC, size until 10 nm, and 1–5 water layers), the improved formulation (3HFWC-W, size 10–25 nm, and 6–9 water layers) showed multiplied cytotoxic activity against melanoma cell lines of different aggressiveness. Apart from this, the mode of action was preserved and based on an induction of senescence rather than cell death. Importantly, high selectivity towards malignant phenotypes was detected. Observed effects can be ascribed to a machinery of hydrogen bonds, which are generated in SD-C60 and transmitted through water to biomolecules. This approach may open a new field in science and healthcare—a “water-based nanomedicine”.
PB  - MDPI
T2  - Micromachines
T1  - The Second Derivative of Fullerene C60 (SD-C60) and Biomolecular Machinery of Hydrogen Bonds: Water-Based Nanomedicine
IS  - 14
SP  - 2152
DO  - 10.3390/mi14122152
ER  - 

@article{
author = "Matija, Lidija and Stanković, Ivana and Purić, Milica and Miličić, Milica and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Krajnović, Tamara and Gordić, Vuk and Koruga, Đuro",
year = "2023",
abstract = "The human body contains 60–70% water, depending on age. As a body fluid, it is not only a medium in which physical and chemical processes take place, but it is also one of the active mediators. Water is the richest substance with non-covalent hydrogen bonds. Water molecules, by themselves (in vacuum), are diamagnetic but when organized into clusters, they become diamagnetic or paramagnetic. Also, biomolecules (DNA, collagen, clathrin, and other proteins) have non-covalent hydrogen bonds in their structure. The interaction, as well as signal transmission, between water and biomolecules is achieved through the vibrations of covalent and non-covalent hydrogen bonds, which determine the state and dynamics of conformational changes in biomolecules. Disruptive conformational changes in biomolecules, cells, and tissues lead to their dysfunctionality, so they are a frequent cause of many disorders and diseases. For example, the rearrangement of hydrogen bonding due to mitochondrial disease mutation in cytochrome bc1 disturbs heme bH redox potential and spin state. In order to prevent and repair the dysfunctional conformational changes, a liquid substance was developed based on the second derivative of the C60 molecule (SD-C60), which has classical and quantum properties. The characterization of SD-C60 by UV-VIS-NIR, FTIR, TEM, and AFM/MFM was performed and it is shown that SD-C60 water layers generate vibrations with near-zero phase dispersion which are transmitted through Fibonacci’s water chains to biomolecules. In comparison with previously published SD-C60 derivate (3HFWC, size until 10 nm, and 1–5 water layers), the improved formulation (3HFWC-W, size 10–25 nm, and 6–9 water layers) showed multiplied cytotoxic activity against melanoma cell lines of different aggressiveness. Apart from this, the mode of action was preserved and based on an induction of senescence rather than cell death. Importantly, high selectivity towards malignant phenotypes was detected. Observed effects can be ascribed to a machinery of hydrogen bonds, which are generated in SD-C60 and transmitted through water to biomolecules. This approach may open a new field in science and healthcare—a “water-based nanomedicine”.",
publisher = "MDPI",
journal = "Micromachines",
title = "The Second Derivative of Fullerene C60 (SD-C60) and Biomolecular Machinery of Hydrogen Bonds: Water-Based Nanomedicine",
number = "14",
pages = "2152",
doi = "10.3390/mi14122152"
}

Matija, L., Stanković, I., Purić, M., Miličić, M., Maksimović-Ivanić, D., Mijatović, S., Krajnović, T., Gordić, V.,& Koruga, Đ.. (2023). The Second Derivative of Fullerene C60 (SD-C60) and Biomolecular Machinery of Hydrogen Bonds: Water-Based Nanomedicine. in Micromachines
MDPI.(14), 2152.
https://doi.org/10.3390/mi14122152

Matija L, Stanković I, Purić M, Miličić M, Maksimović-Ivanić D, Mijatović S, Krajnović T, Gordić V, Koruga Đ. The Second Derivative of Fullerene C60 (SD-C60) and Biomolecular Machinery of Hydrogen Bonds: Water-Based Nanomedicine. in Micromachines. 2023;(14):2152.
doi:10.3390/mi14122152 .

Matija, Lidija, Stanković, Ivana, Purić, Milica, Miličić, Milica, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Krajnović, Tamara, Gordić, Vuk, Koruga, Đuro, "The Second Derivative of Fullerene C60 (SD-C60) and Biomolecular Machinery of Hydrogen Bonds: Water-Based Nanomedicine" in Micromachines, no. 14 (2023):2152,
https://doi.org/10.3390/mi14122152 . .

TY  - JOUR
AU  - Antonijević, Đorđe
AU  - Despotović, Ana
AU  - Biocanin, Vladimir
AU  - Milošević, Miloš
AU  - Trisić, Dijana
AU  - Lazović, Vladimir
AU  - Zogović, Nevena
AU  - Milasin, Jelena
AU  - Ilić, Dragan
PY  - 2021
UR  - https://machinery.mas.bg.ac.rs/handle/123456789/3492
AB  - The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nanoparticulated hydroxyapatite - ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p  lt  0.05). Both initial and final setting times as well as compressive strengths were statistically lower for experimental cements than for PC and MTA (p  lt  0.05). The lowest total porosity was observed in the ECHA + ZrO2 group when compared with the other two experimental cements (p  lt  0.05), but not when compared with PC and MTA (p > 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p  lt  0.05). For blood plasma, a statistical difference was found only between ECHA + Bi2O3 and PC (p  lt  0.05). All investigated materials had alkalization ability. Cell viability assays revealed that the extracts of tested cements did not exhibit cytotoxic effect on L929 cells. Scanning electron microscopy had shown a high degree of cell proliferation and adhesion of cells from apical papilla on experimental cements' surfaces. Novel endodontic ceramics with nano-hydroxyapatite addition have satisfactory biological and physicochemical properties when compared to MTA and PC controls. Considerable lower setting time of experimental cements might present a huge advantage of these synthesized materials in clinical practice. SrF2 presents a novel promising radiopacifying agent for dental cements manufacturing.
PB  - Elsevier Sci Ltd, Oxford
T2  - Ceramics International
T1  - Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic
EP  - 28923
IS  - 20
SP  - 28913
VL  - 47
DO  - 10.1016/j.ceramint.2021.07.052
ER  - 

@article{
author = "Antonijević, Đorđe and Despotović, Ana and Biocanin, Vladimir and Milošević, Miloš and Trisić, Dijana and Lazović, Vladimir and Zogović, Nevena and Milasin, Jelena and Ilić, Dragan",
year = "2021",
abstract = "The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nanoparticulated hydroxyapatite - ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p  lt  0.05). Both initial and final setting times as well as compressive strengths were statistically lower for experimental cements than for PC and MTA (p  lt  0.05). The lowest total porosity was observed in the ECHA + ZrO2 group when compared with the other two experimental cements (p  lt  0.05), but not when compared with PC and MTA (p > 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p  lt  0.05). For blood plasma, a statistical difference was found only between ECHA + Bi2O3 and PC (p  lt  0.05). All investigated materials had alkalization ability. Cell viability assays revealed that the extracts of tested cements did not exhibit cytotoxic effect on L929 cells. Scanning electron microscopy had shown a high degree of cell proliferation and adhesion of cells from apical papilla on experimental cements' surfaces. Novel endodontic ceramics with nano-hydroxyapatite addition have satisfactory biological and physicochemical properties when compared to MTA and PC controls. Considerable lower setting time of experimental cements might present a huge advantage of these synthesized materials in clinical practice. SrF2 presents a novel promising radiopacifying agent for dental cements manufacturing.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Ceramics International",
title = "Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic",
pages = "28923-28913",
number = "20",
volume = "47",
doi = "10.1016/j.ceramint.2021.07.052"
}

Antonijević, Đ., Despotović, A., Biocanin, V., Milošević, M., Trisić, D., Lazović, V., Zogović, N., Milasin, J.,& Ilić, D.. (2021). Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic. in Ceramics International
Elsevier Sci Ltd, Oxford., 47(20), 28913-28923.
https://doi.org/10.1016/j.ceramint.2021.07.052

Antonijević Đ, Despotović A, Biocanin V, Milošević M, Trisić D, Lazović V, Zogović N, Milasin J, Ilić D. Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic. in Ceramics International. 2021;47(20):28913-28923.
doi:10.1016/j.ceramint.2021.07.052 .

Antonijević, Đorđe, Despotović, Ana, Biocanin, Vladimir, Milošević, Miloš, Trisić, Dijana, Lazović, Vladimir, Zogović, Nevena, Milasin, Jelena, Ilić, Dragan, "Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic" in Ceramics International, 47, no. 20 (2021):28913-28923,
https://doi.org/10.1016/j.ceramint.2021.07.052 . .