TY  - JOUR
AU  - Milasinović, Nikola
AU  - Knežević-Jugović, Zorica
AU  - Jakovljević, Živana
AU  - Filipović, Jovanka
AU  - Kalagasidis-Krusić, Melina
PY  - 2012
UR  - https://machinery.mas.bg.ac.rs/handle/123456789/1367
AB  - A commercial Candida rugosa lipase was immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogel under different pH immobilization conditions, characterized and evaluated for both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium. Although the apparent enzyme activity in the hydrolytic reaction was acceptable, the immobilized lipase appeared to be more suitable for application in ester synthesis in low aqueous system based on n-hexane. The esterification reaction parameters, such as temperature, biocatalyst amount, added water content and substrate concentration was evaluated and optimized by response surface analysis. An adequate statistical quadratic model was obtained, making it possible to predict ester yields from known values of the four main factors. Under optimized conditions, the yield of ester >90% could be achieved after 48 h of reaction, corresponding to the volumetric activity of 4.75 mmol L-1 h(-1). The operational stability of the immobilized system in esterification reaction proved to be highly attractive with 15 consecutive 48 h uses with a residual activity of 67.4%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.
PB  - Elsevier Science Sa, Lausanne
T2  - Chemical Engineering Journal
T1  - Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels
EP  - 623
SP  - 614
VL  - 181
DO  - 10.1016/j.cej.2011.11.115
ER  - 

@article{
author = "Milasinović, Nikola and Knežević-Jugović, Zorica and Jakovljević, Živana and Filipović, Jovanka and Kalagasidis-Krusić, Melina",
year = "2012",
abstract = "A commercial Candida rugosa lipase was immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogel under different pH immobilization conditions, characterized and evaluated for both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium. Although the apparent enzyme activity in the hydrolytic reaction was acceptable, the immobilized lipase appeared to be more suitable for application in ester synthesis in low aqueous system based on n-hexane. The esterification reaction parameters, such as temperature, biocatalyst amount, added water content and substrate concentration was evaluated and optimized by response surface analysis. An adequate statistical quadratic model was obtained, making it possible to predict ester yields from known values of the four main factors. Under optimized conditions, the yield of ester >90% could be achieved after 48 h of reaction, corresponding to the volumetric activity of 4.75 mmol L-1 h(-1). The operational stability of the immobilized system in esterification reaction proved to be highly attractive with 15 consecutive 48 h uses with a residual activity of 67.4%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Chemical Engineering Journal",
title = "Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels",
pages = "623-614",
volume = "181",
doi = "10.1016/j.cej.2011.11.115"
}

Milasinović, N., Knežević-Jugović, Z., Jakovljević, Ž., Filipović, J.,& Kalagasidis-Krusić, M.. (2012). Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels. in Chemical Engineering Journal
Elsevier Science Sa, Lausanne., 181, 614-623.
https://doi.org/10.1016/j.cej.2011.11.115

Milasinović N, Knežević-Jugović Z, Jakovljević Ž, Filipović J, Kalagasidis-Krusić M. Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels. in Chemical Engineering Journal. 2012;181:614-623.
doi:10.1016/j.cej.2011.11.115 .

Milasinović, Nikola, Knežević-Jugović, Zorica, Jakovljević, Živana, Filipović, Jovanka, Kalagasidis-Krusić, Melina, "Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels" in Chemical Engineering Journal, 181 (2012):614-623,
https://doi.org/10.1016/j.cej.2011.11.115 . .