Effect of Acetylsalicylic Acid on Biological Properties of Novel Cement Based on Calcium Phosphate Doped with Ions of Strontium, Copper, and Zinc
2024
Authors
Vlajić Tovilović, TamaraPetrović, Sanja
Lazarević, Miloš
Pavić, Aleksandar
Plačkić, Nikola
Milovanović, Aleksa
Milošević, Miloš
Miletić, Vesna
Veljović, Đorđe
Radunović, Milena
Article (Published version)
Metadata
Show full item recordAbstract
This study aimed to compare the biological properties of newly synthesized cements
based on calcium phosphate with a commercially used cement, mineral trioxide aggregate (MTA).
Strontium (Sr)-, Copper (Cu)-, and Zinc (Zn)-doped hydroxyapatite (miHAp) powder was obtained
through hydrothermal synthesis and characterized by scanning electron microscopy (SEM), X-ray
diffraction (XRD), and energy dispersive X-ray spectrometry (EDX). Calcium phosphate cement
(CPC) was produced by mixing miHAp powder with a 20 wt.% citric acid solution, followed by the
assessment of its compressive strength, setting time, and in vitro bioactivity. Acetylsalicylic acid
(ASA) was added to the CPC, resulting in CPCA. Biological tests were conducted on CPC, CPCA,
and MTA. The biocompatibility of the cement extracts was evaluated in vitro using human dental
pulp stem cells (hDPSCs) and in vivo using a zebrafish model. Antibiofilm and antimicrobial effect
(quantified by CFUs/mL) were assessed against Stre...ptococcus mutans and Lactobacillus rhamnosus.
None of the tested materials showed toxicity, while CPCA even increased hDPSCs proliferation.
CPCA showed a better safety profile than MTA and CPC, and no toxic or immunomodulatory
effects on the zebrafish model. CPCA exhibited similar antibiofilm effects against S. mutans and L.
rhamnosus to MTA.
Keywords:
calcium phosphate / hydroxyapatite / antibiofilm / dental pulp stem cells / zebrafishSource:
International Journal of Molecular Sciences, 2024, 25, 14, 7940-Publisher:
- MDPI
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200135)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200129 (University of Belgrade, Faculty of Dental Medicine) (RS-MESTD-inst-2020-200129)
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Institution/Community
Inovacioni centarTY - JOUR AU - Vlajić Tovilović, Tamara AU - Petrović, Sanja AU - Lazarević, Miloš AU - Pavić, Aleksandar AU - Plačkić, Nikola AU - Milovanović, Aleksa AU - Milošević, Miloš AU - Miletić, Vesna AU - Veljović, Đorđe AU - Radunović, Milena PY - 2024 UR - https://machinery.mas.bg.ac.rs/handle/123456789/7894 AB - This study aimed to compare the biological properties of newly synthesized cements based on calcium phosphate with a commercially used cement, mineral trioxide aggregate (MTA). Strontium (Sr)-, Copper (Cu)-, and Zinc (Zn)-doped hydroxyapatite (miHAp) powder was obtained through hydrothermal synthesis and characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectrometry (EDX). Calcium phosphate cement (CPC) was produced by mixing miHAp powder with a 20 wt.% citric acid solution, followed by the assessment of its compressive strength, setting time, and in vitro bioactivity. Acetylsalicylic acid (ASA) was added to the CPC, resulting in CPCA. Biological tests were conducted on CPC, CPCA, and MTA. The biocompatibility of the cement extracts was evaluated in vitro using human dental pulp stem cells (hDPSCs) and in vivo using a zebrafish model. Antibiofilm and antimicrobial effect (quantified by CFUs/mL) were assessed against Streptococcus mutans and Lactobacillus rhamnosus. None of the tested materials showed toxicity, while CPCA even increased hDPSCs proliferation. CPCA showed a better safety profile than MTA and CPC, and no toxic or immunomodulatory effects on the zebrafish model. CPCA exhibited similar antibiofilm effects against S. mutans and L. rhamnosus to MTA. PB - MDPI T2 - International Journal of Molecular Sciences T1 - Effect of Acetylsalicylic Acid on Biological Properties of Novel Cement Based on Calcium Phosphate Doped with Ions of Strontium, Copper, and Zinc IS - 14 SP - 7940 VL - 25 DO - 10.3390/ijms25147940 ER -
@article{ author = "Vlajić Tovilović, Tamara and Petrović, Sanja and Lazarević, Miloš and Pavić, Aleksandar and Plačkić, Nikola and Milovanović, Aleksa and Milošević, Miloš and Miletić, Vesna and Veljović, Đorđe and Radunović, Milena", year = "2024", abstract = "This study aimed to compare the biological properties of newly synthesized cements based on calcium phosphate with a commercially used cement, mineral trioxide aggregate (MTA). Strontium (Sr)-, Copper (Cu)-, and Zinc (Zn)-doped hydroxyapatite (miHAp) powder was obtained through hydrothermal synthesis and characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectrometry (EDX). Calcium phosphate cement (CPC) was produced by mixing miHAp powder with a 20 wt.% citric acid solution, followed by the assessment of its compressive strength, setting time, and in vitro bioactivity. Acetylsalicylic acid (ASA) was added to the CPC, resulting in CPCA. Biological tests were conducted on CPC, CPCA, and MTA. The biocompatibility of the cement extracts was evaluated in vitro using human dental pulp stem cells (hDPSCs) and in vivo using a zebrafish model. Antibiofilm and antimicrobial effect (quantified by CFUs/mL) were assessed against Streptococcus mutans and Lactobacillus rhamnosus. None of the tested materials showed toxicity, while CPCA even increased hDPSCs proliferation. CPCA showed a better safety profile than MTA and CPC, and no toxic or immunomodulatory effects on the zebrafish model. CPCA exhibited similar antibiofilm effects against S. mutans and L. rhamnosus to MTA.", publisher = "MDPI", journal = "International Journal of Molecular Sciences", title = "Effect of Acetylsalicylic Acid on Biological Properties of Novel Cement Based on Calcium Phosphate Doped with Ions of Strontium, Copper, and Zinc", number = "14", pages = "7940", volume = "25", doi = "10.3390/ijms25147940" }
Vlajić Tovilović, T., Petrović, S., Lazarević, M., Pavić, A., Plačkić, N., Milovanović, A., Milošević, M., Miletić, V., Veljović, Đ.,& Radunović, M.. (2024). Effect of Acetylsalicylic Acid on Biological Properties of Novel Cement Based on Calcium Phosphate Doped with Ions of Strontium, Copper, and Zinc. in International Journal of Molecular Sciences MDPI., 25(14), 7940. https://doi.org/10.3390/ijms25147940
Vlajić Tovilović T, Petrović S, Lazarević M, Pavić A, Plačkić N, Milovanović A, Milošević M, Miletić V, Veljović Đ, Radunović M. Effect of Acetylsalicylic Acid on Biological Properties of Novel Cement Based on Calcium Phosphate Doped with Ions of Strontium, Copper, and Zinc. in International Journal of Molecular Sciences. 2024;25(14):7940. doi:10.3390/ijms25147940 .
Vlajić Tovilović, Tamara, Petrović, Sanja, Lazarević, Miloš, Pavić, Aleksandar, Plačkić, Nikola, Milovanović, Aleksa, Milošević, Miloš, Miletić, Vesna, Veljović, Đorđe, Radunović, Milena, "Effect of Acetylsalicylic Acid on Biological Properties of Novel Cement Based on Calcium Phosphate Doped with Ions of Strontium, Copper, and Zinc" in International Journal of Molecular Sciences, 25, no. 14 (2024):7940, https://doi.org/10.3390/ijms25147940 . .