Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic
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2021
Authors
Antonijević, ĐorđeDespotović, Ana
Biocanin, Vladimir
Milošević, Miloš
Trisić, Dijana
Lazović, Vladimir
Zogović, Nevena
Milasin, Jelena
Ilić, Dragan
Article (Published version)
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The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nanoparticulated hydroxyapatite - ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p lt 0.05). Both initial and final setting times... as well as compressive strengths were statistically lower for experimental cements than for PC and MTA (p lt 0.05). The lowest total porosity was observed in the ECHA + ZrO2 group when compared with the other two experimental cements (p lt 0.05), but not when compared with PC and MTA (p > 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p lt 0.05). For blood plasma, a statistical difference was found only between ECHA + Bi2O3 and PC (p lt 0.05). All investigated materials had alkalization ability. Cell viability assays revealed that the extracts of tested cements did not exhibit cytotoxic effect on L929 cells. Scanning electron microscopy had shown a high degree of cell proliferation and adhesion of cells from apical papilla on experimental cements' surfaces. Novel endodontic ceramics with nano-hydroxyapatite addition have satisfactory biological and physicochemical properties when compared to MTA and PC controls. Considerable lower setting time of experimental cements might present a huge advantage of these synthesized materials in clinical practice. SrF2 presents a novel promising radiopacifying agent for dental cements manufacturing.
Keywords:
Zirconium dioxide / Strontium fluoride / Radiopacifier / Hydroxyapatite / Endodontic surgery / Bismuth oxideSource:
Ceramics International, 2021, 47, 20, 28913-28923Publisher:
- Elsevier Sci Ltd, Oxford
Funding / projects:
- Ministry of Education, Science and Technological Development of the Republic of Serbia [451-02-818/2021-09/20]
- Ministry of Science and Technology of The Peoples Republic of China [451-02-818/2021-09/20]
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča) (RS-MESTD-inst-2020-200017)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
DOI: 10.1016/j.ceramint.2021.07.052
ISSN: 0272-8842
WoS: 000694740300001
Scopus: 2-s2.0-85110294216
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Inovacioni centarTY - JOUR AU - Antonijević, Đorđe AU - Despotović, Ana AU - Biocanin, Vladimir AU - Milošević, Miloš AU - Trisić, Dijana AU - Lazović, Vladimir AU - Zogović, Nevena AU - Milasin, Jelena AU - Ilić, Dragan PY - 2021 UR - https://machinery.mas.bg.ac.rs/handle/123456789/3492 AB - The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nanoparticulated hydroxyapatite - ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p lt 0.05). Both initial and final setting times as well as compressive strengths were statistically lower for experimental cements than for PC and MTA (p lt 0.05). The lowest total porosity was observed in the ECHA + ZrO2 group when compared with the other two experimental cements (p lt 0.05), but not when compared with PC and MTA (p > 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p lt 0.05). For blood plasma, a statistical difference was found only between ECHA + Bi2O3 and PC (p lt 0.05). All investigated materials had alkalization ability. Cell viability assays revealed that the extracts of tested cements did not exhibit cytotoxic effect on L929 cells. Scanning electron microscopy had shown a high degree of cell proliferation and adhesion of cells from apical papilla on experimental cements' surfaces. Novel endodontic ceramics with nano-hydroxyapatite addition have satisfactory biological and physicochemical properties when compared to MTA and PC controls. Considerable lower setting time of experimental cements might present a huge advantage of these synthesized materials in clinical practice. SrF2 presents a novel promising radiopacifying agent for dental cements manufacturing. PB - Elsevier Sci Ltd, Oxford T2 - Ceramics International T1 - Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic EP - 28923 IS - 20 SP - 28913 VL - 47 DO - 10.1016/j.ceramint.2021.07.052 ER -
@article{ author = "Antonijević, Đorđe and Despotović, Ana and Biocanin, Vladimir and Milošević, Miloš and Trisić, Dijana and Lazović, Vladimir and Zogović, Nevena and Milasin, Jelena and Ilić, Dragan", year = "2021", abstract = "The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nanoparticulated hydroxyapatite - ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p lt 0.05). Both initial and final setting times as well as compressive strengths were statistically lower for experimental cements than for PC and MTA (p lt 0.05). The lowest total porosity was observed in the ECHA + ZrO2 group when compared with the other two experimental cements (p lt 0.05), but not when compared with PC and MTA (p > 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p lt 0.05). For blood plasma, a statistical difference was found only between ECHA + Bi2O3 and PC (p lt 0.05). All investigated materials had alkalization ability. Cell viability assays revealed that the extracts of tested cements did not exhibit cytotoxic effect on L929 cells. Scanning electron microscopy had shown a high degree of cell proliferation and adhesion of cells from apical papilla on experimental cements' surfaces. Novel endodontic ceramics with nano-hydroxyapatite addition have satisfactory biological and physicochemical properties when compared to MTA and PC controls. Considerable lower setting time of experimental cements might present a huge advantage of these synthesized materials in clinical practice. SrF2 presents a novel promising radiopacifying agent for dental cements manufacturing.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Ceramics International", title = "Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic", pages = "28923-28913", number = "20", volume = "47", doi = "10.1016/j.ceramint.2021.07.052" }
Antonijević, Đ., Despotović, A., Biocanin, V., Milošević, M., Trisić, D., Lazović, V., Zogović, N., Milasin, J.,& Ilić, D.. (2021). Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic. in Ceramics International Elsevier Sci Ltd, Oxford., 47(20), 28913-28923. https://doi.org/10.1016/j.ceramint.2021.07.052
Antonijević Đ, Despotović A, Biocanin V, Milošević M, Trisić D, Lazović V, Zogović N, Milasin J, Ilić D. Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic. in Ceramics International. 2021;47(20):28913-28923. doi:10.1016/j.ceramint.2021.07.052 .
Antonijević, Đorđe, Despotović, Ana, Biocanin, Vladimir, Milošević, Miloš, Trisić, Dijana, Lazović, Vladimir, Zogović, Nevena, Milasin, Jelena, Ilić, Dragan, "Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic" in Ceramics International, 47, no. 20 (2021):28913-28923, https://doi.org/10.1016/j.ceramint.2021.07.052 . .