DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells
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2018
Authors
Dojčilović, RadovanPajović, Jelena D.
Božanić, Dušan K.
Jović, Nataša
Pavlović, Vera P.
Pavlović, Vladimir B.
Acković, Lea Lenhardt
Zeković, Ivana
Dramićanin, Miroslav
Kaščakova, Slavka
Refregiers, Matthieu
Rašić, Goran
Vlahović, Branislav
Djoković, Vladimir
Article (Published version)
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Show full item recordAbstract
The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp(2) and sp(3) carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, p...rGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by similar to 2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery.
Keywords:
graphene oxide / fluorescence / cells / cancer / bioimagingSource:
2D Materials, 2018, 5, 4, 045019-Publisher:
- IOP Publishing Ltd, Bristol
Funding / projects:
- NSF CREST [HRD-0833184]
- NASA [NNX09AV07A]
- Size-, shape- and structure- dependent properties of nanoparticles and nanocomposites (RS-MESTD-Basic Research (BR or ON)-172056)
- Materials of Reduced Dimensions for Efficient Light Harvesting and Energy conversion (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45020)
- Investigation of the effect of parameters of synthesis and processing on dielectric, optical and magnetic properties, both bulk and surface of crystal and polymeric systems (RS-MESTD-Basic Research (BR or ON)-171029)
Note:
- Peer reviewed version of the paper: https://machinery.mas.bg.ac.rs/handle/123456789/4042
Related info:
DOI: 10.1088/2053-1583/aad72b
ISSN: 2053-1583
WoS: 000441754800003
Scopus: 2-s2.0-85054724220
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Mašinski fakultetTY - JOUR AU - Dojčilović, Radovan AU - Pajović, Jelena D. AU - Božanić, Dušan K. AU - Jović, Nataša AU - Pavlović, Vera P. AU - Pavlović, Vladimir B. AU - Acković, Lea Lenhardt AU - Zeković, Ivana AU - Dramićanin, Miroslav AU - Kaščakova, Slavka AU - Refregiers, Matthieu AU - Rašić, Goran AU - Vlahović, Branislav AU - Djoković, Vladimir PY - 2018 UR - https://machinery.mas.bg.ac.rs/handle/123456789/2868 AB - The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp(2) and sp(3) carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by similar to 2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery. PB - IOP Publishing Ltd, Bristol T2 - 2D Materials T1 - DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells IS - 4 SP - 045019 VL - 5 DO - 10.1088/2053-1583/aad72b ER -
@article{ author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Jović, Nataša and Pavlović, Vera P. and Pavlović, Vladimir B. and Acković, Lea Lenhardt and Zeković, Ivana and Dramićanin, Miroslav and Kaščakova, Slavka and Refregiers, Matthieu and Rašić, Goran and Vlahović, Branislav and Djoković, Vladimir", year = "2018", abstract = "The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp(2) and sp(3) carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by similar to 2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery.", publisher = "IOP Publishing Ltd, Bristol", journal = "2D Materials", title = "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells", number = "4", pages = "045019", volume = "5", doi = "10.1088/2053-1583/aad72b" }
Dojčilović, R., Pajović, J. D., Božanić, D. K., Jović, N., Pavlović, V. P., Pavlović, V. B., Acković, L. L., Zeković, I., Dramićanin, M., Kaščakova, S., Refregiers, M., Rašić, G., Vlahović, B.,& Djoković, V.. (2018). DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2D Materials IOP Publishing Ltd, Bristol., 5(4), 045019. https://doi.org/10.1088/2053-1583/aad72b
Dojčilović R, Pajović JD, Božanić DK, Jović N, Pavlović VP, Pavlović VB, Acković LL, Zeković I, Dramićanin M, Kaščakova S, Refregiers M, Rašić G, Vlahović B, Djoković V. DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2D Materials. 2018;5(4):045019. doi:10.1088/2053-1583/aad72b .
Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Jović, Nataša, Pavlović, Vera P., Pavlović, Vladimir B., Acković, Lea Lenhardt, Zeković, Ivana, Dramićanin, Miroslav, Kaščakova, Slavka, Refregiers, Matthieu, Rašić, Goran, Vlahović, Branislav, Djoković, Vladimir, "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells" in 2D Materials, 5, no. 4 (2018):045019, https://doi.org/10.1088/2053-1583/aad72b . .