Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels
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2012
Authors
Milasinović, NikolaKnežević-Jugović, Zorica
Jakovljević, Živana
Filipović, Jovanka
Kalagasidis-Krusić, Melina
Article (Published version)
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Show full item recordAbstract
A commercial Candida rugosa lipase was immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogel under different pH immobilization conditions, characterized and evaluated for both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium. Although the apparent enzyme activity in the hydrolytic reaction was acceptable, the immobilized lipase appeared to be more suitable for application in ester synthesis in low aqueous system based on n-hexane. The esterification reaction parameters, such as temperature, biocatalyst amount, added water content and substrate concentration was evaluated and optimized by response surface analysis. An adequate statistical quadratic model was obtained, making it possible to predict ester yields from known values of the four main factors. Under optimized conditions, the yield of ester >90% could be achieved after 48 h of reaction, corresponding to the volumetric activity of 4.75 mmol L-1 h(-1). The operational stabili...ty of the immobilized system in esterification reaction proved to be highly attractive with 15 consecutive 48 h uses with a residual activity of 67.4%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.
Keywords:
N-Isopropylacrylamide / Itaconic acid / Ester synthesis / Enzyme immobilization / Candida rugosa lipaseSource:
Chemical Engineering Journal, 2012, 181, 614-623Publisher:
- Elsevier Science Sa, Lausanne
Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Synthesis and characterization of novel functional polymers and polymeric nanocomposites (RS-MESTD-Basic Research (BR or ON)-172062)
DOI: 10.1016/j.cej.2011.11.115
ISSN: 1385-8947
WoS: 000301087000069
Scopus: 2-s2.0-84856318182
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Mašinski fakultetTY - JOUR AU - Milasinović, Nikola AU - Knežević-Jugović, Zorica AU - Jakovljević, Živana AU - Filipović, Jovanka AU - Kalagasidis-Krusić, Melina PY - 2012 UR - https://machinery.mas.bg.ac.rs/handle/123456789/1367 AB - A commercial Candida rugosa lipase was immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogel under different pH immobilization conditions, characterized and evaluated for both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium. Although the apparent enzyme activity in the hydrolytic reaction was acceptable, the immobilized lipase appeared to be more suitable for application in ester synthesis in low aqueous system based on n-hexane. The esterification reaction parameters, such as temperature, biocatalyst amount, added water content and substrate concentration was evaluated and optimized by response surface analysis. An adequate statistical quadratic model was obtained, making it possible to predict ester yields from known values of the four main factors. Under optimized conditions, the yield of ester >90% could be achieved after 48 h of reaction, corresponding to the volumetric activity of 4.75 mmol L-1 h(-1). The operational stability of the immobilized system in esterification reaction proved to be highly attractive with 15 consecutive 48 h uses with a residual activity of 67.4%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale. PB - Elsevier Science Sa, Lausanne T2 - Chemical Engineering Journal T1 - Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels EP - 623 SP - 614 VL - 181 DO - 10.1016/j.cej.2011.11.115 ER -
@article{ author = "Milasinović, Nikola and Knežević-Jugović, Zorica and Jakovljević, Živana and Filipović, Jovanka and Kalagasidis-Krusić, Melina", year = "2012", abstract = "A commercial Candida rugosa lipase was immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogel under different pH immobilization conditions, characterized and evaluated for both hydrolytic activity in an aqueous medium and esterolytic activity in an organic medium. Although the apparent enzyme activity in the hydrolytic reaction was acceptable, the immobilized lipase appeared to be more suitable for application in ester synthesis in low aqueous system based on n-hexane. The esterification reaction parameters, such as temperature, biocatalyst amount, added water content and substrate concentration was evaluated and optimized by response surface analysis. An adequate statistical quadratic model was obtained, making it possible to predict ester yields from known values of the four main factors. Under optimized conditions, the yield of ester >90% could be achieved after 48 h of reaction, corresponding to the volumetric activity of 4.75 mmol L-1 h(-1). The operational stability of the immobilized system in esterification reaction proved to be highly attractive with 15 consecutive 48 h uses with a residual activity of 67.4%, implying that the developed hydrogel and immobilized system could provide a promising solution for the flavor ester synthesis at the industrial scale.", publisher = "Elsevier Science Sa, Lausanne", journal = "Chemical Engineering Journal", title = "Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels", pages = "623-614", volume = "181", doi = "10.1016/j.cej.2011.11.115" }
Milasinović, N., Knežević-Jugović, Z., Jakovljević, Ž., Filipović, J.,& Kalagasidis-Krusić, M.. (2012). Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels. in Chemical Engineering Journal Elsevier Science Sa, Lausanne., 181, 614-623. https://doi.org/10.1016/j.cej.2011.11.115
Milasinović N, Knežević-Jugović Z, Jakovljević Ž, Filipović J, Kalagasidis-Krusić M. Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels. in Chemical Engineering Journal. 2012;181:614-623. doi:10.1016/j.cej.2011.11.115 .
Milasinović, Nikola, Knežević-Jugović, Zorica, Jakovljević, Živana, Filipović, Jovanka, Kalagasidis-Krusić, Melina, "Synthesis of n-amyl isobutyrate catalyzed by Candida rugosa lipase immobilized into poly(N-isopropylacrylamide-co-itaconic acid) hydrogels" in Chemical Engineering Journal, 181 (2012):614-623, https://doi.org/10.1016/j.cej.2011.11.115 . .