TY  - JOUR
AU  - Dojčilović, Radovan
AU  - Pajović, Jelena D.
AU  - Božanić, Dušan K.
AU  - Jović, Nataša
AU  - Pavlović, Vera P.
AU  - Pavlović, Vladimir B.
AU  - Acković, Lea Lenhardt
AU  - Zeković, Ivana
AU  - Dramićanin, Miroslav
AU  - Kaščakova, Slavka
AU  - Refregiers, Matthieu
AU  - Rašić, Goran
AU  - Vlahović, Branislav
AU  - Djoković, Vladimir
PY  - 2018
UR  - https://machinery.mas.bg.ac.rs/handle/123456789/2868
AB  - The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp(2) and sp(3) carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by similar to 2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery.
PB  - IOP Publishing Ltd, Bristol
T2  - 2D Materials
T1  - DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells
IS  - 4
SP  - 045019
VL  - 5
DO  - 10.1088/2053-1583/aad72b
ER  - 

@article{
author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Jović, Nataša and Pavlović, Vera P. and Pavlović, Vladimir B. and Acković, Lea Lenhardt and Zeković, Ivana and Dramićanin, Miroslav and Kaščakova, Slavka and Refregiers, Matthieu and Rašić, Goran and Vlahović, Branislav and Djoković, Vladimir",
year = "2018",
abstract = "The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp(2) and sp(3) carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by similar to 2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery.",
publisher = "IOP Publishing Ltd, Bristol",
journal = "2D Materials",
title = "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells",
number = "4",
pages = "045019",
volume = "5",
doi = "10.1088/2053-1583/aad72b"
}

Dojčilović, R., Pajović, J. D., Božanić, D. K., Jović, N., Pavlović, V. P., Pavlović, V. B., Acković, L. L., Zeković, I., Dramićanin, M., Kaščakova, S., Refregiers, M., Rašić, G., Vlahović, B.,& Djoković, V.. (2018). DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2D Materials
IOP Publishing Ltd, Bristol., 5(4), 045019.
https://doi.org/10.1088/2053-1583/aad72b

Dojčilović R, Pajović JD, Božanić DK, Jović N, Pavlović VP, Pavlović VB, Acković LL, Zeković I, Dramićanin M, Kaščakova S, Refregiers M, Rašić G, Vlahović B, Djoković V. DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2D Materials. 2018;5(4):045019.
doi:10.1088/2053-1583/aad72b .

Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Jović, Nataša, Pavlović, Vera P., Pavlović, Vladimir B., Acković, Lea Lenhardt, Zeković, Ivana, Dramićanin, Miroslav, Kaščakova, Slavka, Refregiers, Matthieu, Rašić, Goran, Vlahović, Branislav, Djoković, Vladimir, "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells" in 2D Materials, 5, no. 4 (2018):045019,
https://doi.org/10.1088/2053-1583/aad72b . .

TY  - JOUR
AU  - Dojčilović, Radovan
AU  - Pajović, Jelena D.
AU  - Božanić, Dušan K.
AU  - Jović, Nataša
AU  - Pavlović, Vera P.
AU  - Pavlović, Vladimir B.
AU  - Acković, Lea Lenhardt
AU  - Zeković, Ivana
AU  - Dramićanin, Miroslav 
AU  - Kaščakova, Slavka
AU  - Refregiers, Matthieu
AU  - Rašić, Goran
AU  - Vlahović, Branislav
AU  - Djoković, Vladimir
PY  - 2018
UR  - https://machinery.mas.bg.ac.rs/handle/123456789/4042
AB  - The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp2 and sp3 carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by ~2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery.
PB  - IOP Publishing Ltd
T2  - 2D Materials
T1  - DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells
IS  - 4
SP  - 045019
VL  - 5
DO  - 10.1088/2053-1583/aad72b
ER  - 

@article{
author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Jović, Nataša and Pavlović, Vera P. and Pavlović, Vladimir B. and Acković, Lea Lenhardt and Zeković, Ivana and Dramićanin, Miroslav  and Kaščakova, Slavka and Refregiers, Matthieu and Rašić, Goran and Vlahović, Branislav and Djoković, Vladimir",
year = "2018",
abstract = "The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp2 and sp3 carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by ~2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery.",
publisher = "IOP Publishing Ltd",
journal = "2D Materials",
title = "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells",
number = "4",
pages = "045019",
volume = "5",
doi = "10.1088/2053-1583/aad72b"
}

Dojčilović, R., Pajović, J. D., Božanić, D. K., Jović, N., Pavlović, V. P., Pavlović, V. B., Acković, L. L., Zeković, I., Dramićanin, M., Kaščakova, S., Refregiers, M., Rašić, G., Vlahović, B.,& Djoković, V.. (2018). DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2D Materials
IOP Publishing Ltd., 5(4), 045019.
https://doi.org/10.1088/2053-1583/aad72b

Dojčilović R, Pajović JD, Božanić DK, Jović N, Pavlović VP, Pavlović VB, Acković LL, Zeković I, Dramićanin M, Kaščakova S, Refregiers M, Rašić G, Vlahović B, Djoković V. DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2D Materials. 2018;5(4):045019.
doi:10.1088/2053-1583/aad72b .

Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Jović, Nataša, Pavlović, Vera P., Pavlović, Vladimir B., Acković, Lea Lenhardt, Zeković, Ivana, Dramićanin, Miroslav , Kaščakova, Slavka, Refregiers, Matthieu, Rašić, Goran, Vlahović, Branislav, Djoković, Vladimir, "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells" in 2D Materials, 5, no. 4 (2018):045019,
https://doi.org/10.1088/2053-1583/aad72b . .

TY  - JOUR
AU  - Manojlović, Dragica
AU  - Dramićanin, Miroslav 
AU  - Milošević, Miloš
AU  - Zeković, Ivana
AU  - Cvijović-Alagić, Ivana
AU  - Mitrović, Nenad
AU  - Miletić, Vesna
PY  - 2016
UR  - https://machinery.mas.bg.ac.rs/handle/123456789/2420
AB  - This study investigated the degree of conversion, depth of cure, Vickers hardness, flexural strength, flexural modulus and volumetric shrinkage of experimental composite containing a low shrinkage monomer FIT-852 (FIT; Esstech Inc.) and photoinitiator 2,4,6-trimethylbenzoyldiphenylphosphine oxide (TPO; Sigma Aldrich) compared to conventional composite containing Bisphenol A-glycidyl methacrylate (BisGMA) and camphorquinone-amine photoinitiator system. The degree of conversion was generally higher in FIT-based composites (45-64% range) than in BisGMA-based composites (34-58% range). Vickers hardness, flexural strength and modulus were higher in BisGMA-based composites. A polywave light-curing unit was generally more efficient in terms of conversion and hardness of experimental composites than a monowave unit. FIT-based composite containing TPO showed the depth of cure below 2 mm irrespective of the curing light. The depth of cure of FIT-based composite containing CQ and BisGMA-based composites with either photoinitiator was in the range of 2.8-3.0 mm. Volumetric shrinkage of FIT-based composite (0.9-5.7% range) was lower than that of BisGMA-based composite (2.2-12% range). FIT may be used as a shrinkage reducing monomer compatible with the conventional CQ-amine system as well as the alternative TPO photoinitiator. However, the depth of cure of FIT_TPO composite requires boosting to achieve clinically recommended thickness of 2 mm.
PB  - Elsevier, Amsterdam
T2  - Materials Science & Engineering C-Materials For Biological Applications
T1  - Effects of a low-Shrinkage methacrylate monomer and monoacylphosphine oxide photoinitiator on curing efficiency and mechanical properties of experimental resin-based composites
EP  - 494
SP  - 487
VL  - 58
DO  - 10.1016/j.msec.2015.08.054
ER  - 

@article{
author = "Manojlović, Dragica and Dramićanin, Miroslav  and Milošević, Miloš and Zeković, Ivana and Cvijović-Alagić, Ivana and Mitrović, Nenad and Miletić, Vesna",
year = "2016",
abstract = "This study investigated the degree of conversion, depth of cure, Vickers hardness, flexural strength, flexural modulus and volumetric shrinkage of experimental composite containing a low shrinkage monomer FIT-852 (FIT; Esstech Inc.) and photoinitiator 2,4,6-trimethylbenzoyldiphenylphosphine oxide (TPO; Sigma Aldrich) compared to conventional composite containing Bisphenol A-glycidyl methacrylate (BisGMA) and camphorquinone-amine photoinitiator system. The degree of conversion was generally higher in FIT-based composites (45-64% range) than in BisGMA-based composites (34-58% range). Vickers hardness, flexural strength and modulus were higher in BisGMA-based composites. A polywave light-curing unit was generally more efficient in terms of conversion and hardness of experimental composites than a monowave unit. FIT-based composite containing TPO showed the depth of cure below 2 mm irrespective of the curing light. The depth of cure of FIT-based composite containing CQ and BisGMA-based composites with either photoinitiator was in the range of 2.8-3.0 mm. Volumetric shrinkage of FIT-based composite (0.9-5.7% range) was lower than that of BisGMA-based composite (2.2-12% range). FIT may be used as a shrinkage reducing monomer compatible with the conventional CQ-amine system as well as the alternative TPO photoinitiator. However, the depth of cure of FIT_TPO composite requires boosting to achieve clinically recommended thickness of 2 mm.",
publisher = "Elsevier, Amsterdam",
journal = "Materials Science & Engineering C-Materials For Biological Applications",
title = "Effects of a low-Shrinkage methacrylate monomer and monoacylphosphine oxide photoinitiator on curing efficiency and mechanical properties of experimental resin-based composites",
pages = "494-487",
volume = "58",
doi = "10.1016/j.msec.2015.08.054"
}

Manojlović, D., Dramićanin, M., Milošević, M., Zeković, I., Cvijović-Alagić, I., Mitrović, N.,& Miletić, V.. (2016). Effects of a low-Shrinkage methacrylate monomer and monoacylphosphine oxide photoinitiator on curing efficiency and mechanical properties of experimental resin-based composites. in Materials Science & Engineering C-Materials For Biological Applications
Elsevier, Amsterdam., 58, 487-494.
https://doi.org/10.1016/j.msec.2015.08.054

Manojlović D, Dramićanin M, Milošević M, Zeković I, Cvijović-Alagić I, Mitrović N, Miletić V. Effects of a low-Shrinkage methacrylate monomer and monoacylphosphine oxide photoinitiator on curing efficiency and mechanical properties of experimental resin-based composites. in Materials Science & Engineering C-Materials For Biological Applications. 2016;58:487-494.
doi:10.1016/j.msec.2015.08.054 .

Manojlović, Dragica, Dramićanin, Miroslav , Milošević, Miloš, Zeković, Ivana, Cvijović-Alagić, Ivana, Mitrović, Nenad, Miletić, Vesna, "Effects of a low-Shrinkage methacrylate monomer and monoacylphosphine oxide photoinitiator on curing efficiency and mechanical properties of experimental resin-based composites" in Materials Science & Engineering C-Materials For Biological Applications, 58 (2016):487-494,
https://doi.org/10.1016/j.msec.2015.08.054 . .

TY  - JOUR
AU  - Dramićanin, Miroslav 
AU  - Zeković, Ivana
AU  - Dimitrijević, B.
AU  - Ribar, Srđan
AU  - Dramićanin, Miroslav 
PY  - 2009
UR  - https://machinery.mas.bg.ac.rs/handle/123456789/973
AB  - Supervised self-organizing map, a type of artificial neural network, is applied for classification of human breast tissue samples utilizing data obtained from fluorescence landscape measurements. Female breast tissue samples were taken soon after the surgical resection, identified and stored at -80 degrees C until fluorescence measurements. From fluorescence landscapes obtained in UV-VIS region spectral features showing statistically significant differences between malignant and normal samples are identified and further quantified to serve as a training input to neural network. Additional set of samples was used as a test group input to trained network in order to evaluate performance of proposed optical biopsy method. Classification sensitivity of 83.9% and specificity of 88.9% are found.
PB  - Polish Acad Sciences Inst Physics, Warsaw
T2  - Acta Physica Polonica A
T1  - Optical Biopsy Method for Breast Cancer Diagnosis Based on Artificial Neural Network Classification of Fluorescence Landscape Data
EP  - 692
IS  - 4
SP  - 690
VL  - 116
DO  - 10.12693/APhysPolA.116.690
ER  - 

@article{
author = "Dramićanin, Miroslav  and Zeković, Ivana and Dimitrijević, B. and Ribar, Srđan and Dramićanin, Miroslav ",
year = "2009",
abstract = "Supervised self-organizing map, a type of artificial neural network, is applied for classification of human breast tissue samples utilizing data obtained from fluorescence landscape measurements. Female breast tissue samples were taken soon after the surgical resection, identified and stored at -80 degrees C until fluorescence measurements. From fluorescence landscapes obtained in UV-VIS region spectral features showing statistically significant differences between malignant and normal samples are identified and further quantified to serve as a training input to neural network. Additional set of samples was used as a test group input to trained network in order to evaluate performance of proposed optical biopsy method. Classification sensitivity of 83.9% and specificity of 88.9% are found.",
publisher = "Polish Acad Sciences Inst Physics, Warsaw",
journal = "Acta Physica Polonica A",
title = "Optical Biopsy Method for Breast Cancer Diagnosis Based on Artificial Neural Network Classification of Fluorescence Landscape Data",
pages = "692-690",
number = "4",
volume = "116",
doi = "10.12693/APhysPolA.116.690"
}

Dramićanin, M., Zeković, I., Dimitrijević, B., Ribar, S.,& Dramićanin, M.. (2009). Optical Biopsy Method for Breast Cancer Diagnosis Based on Artificial Neural Network Classification of Fluorescence Landscape Data. in Acta Physica Polonica A
Polish Acad Sciences Inst Physics, Warsaw., 116(4), 690-692.
https://doi.org/10.12693/APhysPolA.116.690

Dramićanin M, Zeković I, Dimitrijević B, Ribar S, Dramićanin M. Optical Biopsy Method for Breast Cancer Diagnosis Based on Artificial Neural Network Classification of Fluorescence Landscape Data. in Acta Physica Polonica A. 2009;116(4):690-692.
doi:10.12693/APhysPolA.116.690 .

Dramićanin, Miroslav , Zeković, Ivana, Dimitrijević, B., Ribar, Srđan, Dramićanin, Miroslav , "Optical Biopsy Method for Breast Cancer Diagnosis Based on Artificial Neural Network Classification of Fluorescence Landscape Data" in Acta Physica Polonica A, 116, no. 4 (2009):690-692,
https://doi.org/10.12693/APhysPolA.116.690 . .