@misc{
author = "Papić-Obradović, Milena and Jeftić, Branislava and Dragičević, Aleksandra and Matija, Lidija and Koruga, Đuro",
year = "2014",
abstract = "Background. According to data obtained by the WCRFI (World Cancer Research Fund International), cervical cancer is the fourth most common cancer in women, with 528,000 new cases diagnosed worldwide in 2012. However, most (84%) of cancer occurred in less developed regions (445,000 cases), compared with
more developed regions (83,000).
There are different methods to characterize cervical tissues and detect cancer. One of them is FTIR (Fourier Transform Infrared) spectroscopy as a method that is relatively recently got a new application in the detection of various types of cancer, and as such is being tested in research as a method of screening for
cervical cancer. FTIR detects irregularities of the cells on a molecular level The development of morphological changes which are available for detection under a microscope were preceded to distortion, and are showing the great importance of FTIR spectroscopy for early detection of precancerous lesions.
The second one other method is a Raman spectroscopy whose advantages over other optical methods, includes high spatial resolution, includes high spatial resolution, the use of less harmful NIR radiation, less demanding preparation of the samples and its application to in vivo / in situ measurements. The molecular
structure of proteins and lipids is different between neoplastic and normal tissues and therefore Raman spectroscopy has been considered promising for the diagnosis of cancer. Despite the fact that the IR and Raman spectroscopy will have important clinical applications, they are expensive for preventive, early
detection of epithelial cancers. Therefore it is necessary to develop a cheap and reliable method for screening in general doctor practice offices and clinical diagnostic. These findings bring into focus the need for implementation of the new methods for cervical cancer, notably for screening, monitoring and diagnostics.
Methods: Study included a group of 791 patients; with 1712 tiles obtained by comparative PAP study and a novel optical method OMIS (Opto-magnetic imaging spectroscopy). The OMIS is a nanophysical diagnostic technique based on the interaction of electromagnetic radiation with valence electrons within the
sample material, therefore examining electron properties of matter (covalent bonds, hydrogen bonds, ionelectron interaction, Van der Waals interaction). The OMIS technique has already yielded positive results in diagnosing epithelial tissues such as skin, colon, oral cavity and other biological samples. The method is noninvasive, and applicable both in vitro and in vivo. These advantages provide basis for an excellent performance in the environment of screening testing, where speed, ease of use, accuracy and low costs equally contribute to successful early diagnosis of cancer.
Results: We compared tiles from same patients. The method showed clear differentiation and classification with high sensitivity prepared with the classical staining method for PAP test (642 stained tiles) and the non-stained tiles (149 non-stained tiles) for all PAP groups (for stained tiles: specificity 78,16%, sensitivity 97,92% and accuracy 85,18%). However, the most important classification is between third (inflammation) and forth (cancer in situ) groups. Much better results were found out for non-stained tiles (specificity 100%, sensitivity 74,07% and accuracy 82,50%), than for stained tiles.
Conclusion: Comparing OMIS method with classical PAP test the time for diagnosis is shorter, expenses are less and efficiency increased. The most important advantage of the OMIS is possibility to use non-stain PAP smears, what gives much quicker diagnosis, around 30 times, to compare with the conventional stain PAP smears method.",
publisher = "Southeast European Medical Forum, 2019",
journal = "FIFTH INTERNATIONAL MEDICAL CONGRESS, Challenges & Priorities in Medicine, Calamity & First Aid Medicine",
title = "Opto-magnetic imaging spectroscopy in characterisation of stain and non-stain pap smears:preliminary study of cervical cancer",
pages = "48-47",
url = "https://hdl.handle.net/21.15107/rcub_machinery_5851"
}
